We report five new spectral variants of bright luminescent protein made by concatenation of the brightest luciferase,
NanoLuc, with various color hues of fluorescent proteins. These proteins, which we call enhanced Nano-lanterns (eNLs),
allow five-color live-cell imaging without external light illumination as well as detection of single molecules.
Furthermore, eNL-based Ca<sup>2+</sup> indicators could be used to image long-term Ca<sup>2+</sup> dynamics in iPS-derived cardiomyocytes.