Objective: To investigate the feasibility of Mueller optical coherence tomography (OCT) imaging of myocardial fiber structures at different locations. METHODS: A pig heart was taken as a sample, and the Mueller OCT system was used to perform two-dimensional scanning on different positions of the pig heart test sample, and the collected image data was processed by digital image processing method to observe the basic structure of the myocardium at different positions. RESULTS: The images of the isolated pig heart after scanning by the OCT system were processed by the relevant algorithm. The images of 16 elements in the Mueller matrix of the myocardial structure at different locations were also observed. It can also be seen that there is some different of the myocardial fiber structures in different depth directions. Conclusion: Mueller Optical coherence tomography (OCT) can be successfully used to image myocardial fiber structures at different locations.