Dr. Kevin W. Eliceiri Profile

Dr. Kevin W. Eliceiri

Director at Univ of Wisconsin Madison

SPIE Involvement:

Conference Program Committee | Author | Student Chapter CoAdvisor

Publications (14)

Proceedings Article | 10 September 2019

Fluorescence lifetime-based intrinsic metabolic signatures of microglia cell (Conference Presentation)

Md Abdul Kader Sagar, Kevin Cheng, Jonathan Ouellette, Justin Williams, Jyoti Watters, Kevin Eliceiri

Proc. SPIE. 11099, Light in Nature VII

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Nervous system, Traumatic brain injury, Visualization, In vitro testing, Brain

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Proceedings Article | 14 March 2018

PolScope based imaging and quantification of fibrillar collagen organization (Conference Presentation)

Adib Keikhosravi, Kevin Eliceiri, Yuming Liu

Proc. SPIE. 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV

KEYWORDS: Collagen, Image segmentation, Image registration, Image analysis, Microscopy, Pathology, Cancer, Imaging systems, Second-harmonic generation, Image processing

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SPIE Journal Paper | 22 January 2018

Review of quantitative multiscale imaging of breast cancer

Michael A. Pinkert, Lonie R. Salkowski, Patricia J. Keely, Timothy Hall, Walter Block, Kevin Eliceiri

JMI Vol. 5 Issue 01

KEYWORDS: Breast cancer, Breast, Imaging systems, Tissues, Mammography, Tumors, Magnetic resonance imaging, Image resolution, Cancer, Optical coherence tomography

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Proceedings Article | 19 April 2017

Wavelength dependent SHG imaging and scattering probes of extracellular matrix (ECM) alterations in ovarian cancer (Conference Presentation)

Paul Campagnola, Karissa Tilbury, Kirby Campbell, Kevin Eliceiri, Manish Patankar

Proc. SPIE. 10043, Diagnosis and Treatment of Diseases in the Breast and Reproductive System

KEYWORDS: Tumors, Tissues, Cancer, Scattering, Second-harmonic generation, Collagen, Ovarian cancer, Optical testing, Scatter measurement, Tissue optics

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Proceedings Article | 27 April 2016

Modeling extracellular matrix (ECM) alterations in ovarian cancer by multiphoton excited fabrication of stromal models (Conference Presentation)

Paul Campagnola, Visar Ajeti, Jorge Lara, Kevin Eliceiri, Mansh Patankar

Proc. SPIE. 9711, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX

KEYWORDS: Tumor growth modeling, Ovarian cancer, Optical alignment, Cancer, Tissues, Tumors, Diagnostics and therapeutics, Polymerization, Biomimetics, Collagen

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SPIE Journal Paper | 4 July 2014

Extraction of optical properties and prediction of light distribution in rat brain tissue

Mehdi Azimipour, Ryan Baumgartner, Yuming Liu, Steven Jacques, Kevin Eliceiri, Ramin Pashaie

JBO Vol. 19 Issue 07

KEYWORDS: Tissue optics, Optical properties, Brain, Scattering, Monte Carlo methods, Absorption, Optogenetics, Light scattering, Transmittance, Light

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Predicting the distribution of light inside any turbid media, such as biological tissue, requires detailed information about the optical properties of the medium, including the absorption and scattering coefficients and the anisotropy factor. Particularly, in biophotonic applications where photons directly interact with the tissue, this information translates to system design optimization, precision in light delivery, and minimization of unintended consequences, such as phototoxicity or photobleaching. In recent years, optogenetics has opened up a new area in deep brain stimulation with light and the method is widely adapted by researchers for the study of the brain circuitries and the dynamics of neurological disorders. A key factor for a successful optogenetic stimulation is delivering an adequate amount of light to the targeted brain objects. The adequate amount of light needed to stimulate each brain object is identified by the tissue optical properties as well as the type of opsin expressed in the tissue, wavelength of the light, and the physical dimensions of the targeted area. Therefore, to implement a precise light delivery system for optogenetics, detailed information about the optical properties of the brain tissue and a mathematical model that incorporates all determining factors is needed to find a good estimation of light distribution in the brain. In general, three measurements are required to obtain the optical properties of any tissue, namely diffuse transmitted light, diffuse reflected light, and transmitted ballistic beam. In this report, these parameters were measured in vitro using intact rat brain slices of 500 μm thickness via a two-integrating spheres optical setup. Then, an inverse adding doubling method was used to extract the optical properties of the tissue from the collected data. These experiments were repeated to cover the whole brain tissue with high spatial resolution for the three different cuts (transverse, sagittal, and coronal) and three different wavelengths (405, 532, and 635 nm) in the visible range of the spectrum. A three-dimensional atlas of the rat brain optical properties was constructed based on the experimental measurements. This database was linked to a Monte Carlo toolbox to simulate light distribution in the tissue for different light source configurations.

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SPIE Journal Paper | 9 January 2014

Computational segmentation of collagen fibers from second-harmonic generation images of breast cancer

Jeremy Bredfeldt, Yuming Liu, Carolyn Pehlke, Matthew Conklin, Joseph Szulczewski, David Inman, Patricia Keely, Robert Nowak, Thomas Mackie, Kevin Eliceiri

JBO Vol. 19 Issue 01

KEYWORDS: Optical fibers, Collagen, Image segmentation, Second-harmonic generation, Image filtering, Tissues, Breast cancer, Detection and tracking algorithms, Computed tomography, Optical filters

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SPIE Journal Paper | 12 November 2013

Simultaneous determination of the second-harmonic generation emission directionality and reduced scattering coefficient from three-dimensional imaging of thick tissues

Gunnsteinn Hall, Kevin Eliceiri, Paul Campagnola

JBO Vol. 18 Issue 11

KEYWORDS: Second-harmonic generation, Tissues, Scattering, Monte Carlo methods, Optical testing, Microscopes, Optical properties, Collagen, Tissue optics, 3D image processing

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SPIE Journal Paper | 14 February 2013

Nonlinear optical microscopy and ultrasound imaging of human cervical structure

Lisa Reusch, Helen Feltovich, Lindsey Carlson, Gunnsteinn Hall, Paul Campagnola, Kevin Eliceiri, Timothy Hall

JBO Vol. 18 Issue 03

KEYWORDS: Second-harmonic generation, Collagen, Ultrasonography, Cervix, Optical fibers, Composites, Tissues, Microscopy, Tissue optics, Transducers

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Proceedings Article | 9 February 2012

Second-harmonic generation and fluorescence lifetime imaging microscopy through a rodent mammary imaging window

Pamela Young, Muhammad Nazir, Michael Szulczewski, Patricia Keely, Kevin Eliceiri

Proc. SPIE. 8226, Multiphoton Microscopy in the Biomedical Sciences XII

KEYWORDS: Fluorescence lifetime imaging, Collagen, Tumors, Luminescence, Second-harmonic generation, Microscopy, Cancer, Breast, Mode conditioning cables, In vivo imaging

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Proceedings Article | 13 February 2009

High-resolution wavefront correction in multiphoton microscopy

G. Hall, M. Ren, W. Amos, K. Eliceiri, J. White

Proc. SPIE. 7183, Multiphoton Microscopy in the Biomedical Sciences IX

KEYWORDS: Modulation, Wavefronts, Monochromatic aberrations, Modulators, Multiphoton microscopy, Diffraction, Zernike polynomials, Spatial light modulators, Liquid crystals, Optical filters

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SPIE Journal Paper | 1 May 2008

Nonlinear optical imaging and spectral-lifetime computational analysis of endogenous and exogenous fluorophores in breast cancer

Paolo Provenzano, Curtis Rueden, Steven Trier, Long Yan, Suzanne Ponik, David Inman, Patricia Keely, Kevin Eliceiri

JBO Vol. 13 Issue 03

KEYWORDS: Collagen, Tumors, Second-harmonic generation, Fluorescence lifetime imaging, Visualization, Optical imaging, Luminescence, 3D image processing, Cancer, Imaging systems

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SPIE Journal Paper | 1 March 2007

In vivo multiphoton fluorescence lifetime imaging of protein-bound and free nicotinamide adenine dinucleotide in normal and precancerous epithelia

Melissa Skala, Kristin Riching, Damian Bird, Annette Gendron-Fitzpatrick, Jens Eickhoff, Kevin Eliceiri, Patricia Keely, Nirmala Ramanujam

JBO Vol. 12 Issue 02

KEYWORDS: In vivo imaging, Fluorescence lifetime imaging, Luminescence, Tissues, Multiphoton fluorescence microscopy, Mode conditioning cables, Tumor growth modeling, Biopsy, Statistical analysis, Multiphoton microscopy

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SPIE Journal Paper | 1 July 2003

Analysis of histology specimens using lifetime multiphoton microscopy

Kevin Eliceiri, Ching-Hua Fan, Gary Lyons, John White

JBO Vol. 8 Issue 03

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Pulsed laser operation, Multiphoton microscopy, Tissues, Microscopy, Tissue optics, Sensors, Imaging systems, Veins

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Showing 5 of 14 publications

Conference Committee Involvement (16)

Multiphoton Microscopy in the Biomedical Sciences XX

2 February 2020 | San Francisco, California, United States

Multiscale Imaging and Spectroscopy

1 February 2020 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XIX

3 February 2019 | San Francisco, California, United States

Diseases in the Breast and Reproductive System V

2 February 2019 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XVIII

28 January 2018 | San Francisco, California, United States

Diseases in the Breast and Reproductive System IV

27 January 2018 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XVII

29 January 2017 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XVI

14 February 2016 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XV

8 February 2015 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XIV

2 February 2014 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XIII

3 February 2013 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XII

22 January 2012 | San Francisco, California, United States

Advanced Microscopy Techniques II

22 May 2011 | Munich, Germany

Multiphoton Microscopy in the Biomedical Sciences XI

23 January 2011 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences X

24 January 2010 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences IX

25 January 2009 | San Jose, California, United States

Showing 5 of 16 Conference Committees

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