Prof. Klaus Suhling Profile

Prof. Klaus Suhling

Professor of Physics at Kings College London

SPIE Involvement:

Conference Program Committee | Author

Publications (27)

Proceedings Article | 13 March 2024 Presentation

Advances in wide-field camera-based time-correlated single photon counting fluorescence lifetime imaging

Klaus Suhling, Jakub Nedbal, Louis Obeid Mogridge, Shirwa Awale

Proceedings Volume PC12848, PC1284808 (2024) https://doi.org/10.1117/12.3001927

KEYWORDS: Time correlated single photon counting, Fluorescence lifetime imaging, Cameras, Microscopy, Single photon avalanche diodes, Visualization, Total internal reflection, Resonance energy transfer, Proteins, Picosecond phenomena

Read Abstract +

Proceedings Article | 12 March 2024 Presentation + Paper

Development of widefield time-resolved fluorescence anisotropy imaging (TR-FAIM) using a single photon avalanche diode (SPAD) array

Louis Obeid Mogridge, Jakub Nedbal, Shirwa Awale, Jess Tallis, Emily Johnston, Francesco Mattioli Della Rocca, Susan Rosser, Robert Henderson, Klaus Suhling

Proceedings Volume 12847, 128470J (2024) https://doi.org/10.1117/12.3001710

KEYWORDS: Fluorescence, Single photon avalanche diodes, Fluorescence lifetime imaging, Anisotropy, Viscosity, Yeast, Fluorescence anisotropy, Time correlated single photon counting, Diffusion

Read Abstract +

Proceedings Article | 12 April 2021 Presentation + Paper

Correction of time-resolved SPAD array measurements for accurate single-photon time-resolved biological imaging

Jakub Nedbal, Francesco Mattioli Della Rocca, Richard Walker, Robert Henderson, Klaus Suhling

Proceedings Volume 11721, 117210T (2021) https://doi.org/10.1117/12.2587755

KEYWORDS: Luminescence, Anisotropy, Image sensors, Biological research, Monte Carlo methods, Time correlated photon counting, Microscopy, Data acquisition, Virtual reality, Time metrology

Read Abstract +

Proceedings Article | 20 August 2020 Presentation

Metamaterial-enhanced photoluminescence spectroscopy

Diane Roth, Pavel Ginzburg, Mazhar Nasir, Alexey Krasavin, Klaus Suhling, David Richards, Viktor Podolskiy, Anatoly Zayats

Proceedings Volume 11468, 114680E (2020) https://doi.org/10.1117/12.2567612

KEYWORDS: Luminescence, Spectroscopy, Process control, Electromagnetism, Metamaterials, Composites, Biophysics, Quantum optics, Nanostructures, Resonance energy transfer

Read Abstract +

Proceedings Article | 7 March 2019 Presentation + Paper

Imaging mitochondrial matrix viscosity in live cells via fluorescence lifetime imaging (FLIM) of fluorescent molecular rotors

I. Emilie Steinmark, Arjuna James, Cécile Dreiss, Gokhan Yahioglu, Klaus Suhling

Proceedings Volume 10893, 108930K (2019) https://doi.org/10.1117/12.2508676

KEYWORDS: Fluorescence lifetime imaging, Luminescence, Live cell imaging, Physics, Microscopy, Spectroscopy, Photon counting, Molecules

Read Abstract +

Proceedings Article | 22 February 2019 Paper

Fluorescence lifetime imaging for viscosity and diffusion measurements

Klaus Suhling, Yurema Teijeiro-Gonzalez, I. Emilie Steinmark, Arjuna James, Augoustina Economou, Gokhan Yahioglu, Alix Le Marois, Liisa Hirvonen, Jakub Nedbal, James Levitt, Pei-Hua Chung, Cécile Dreiss, Andrew Beavil, Rebecca Beavil, Elena Ortiz-Zapater, Christian Lorenz, Maddy Parsons, Alessandro Crnjar, Bethan Cornell, Carla Molteni

Proceedings Volume 10882, 1088210 (2019) https://doi.org/10.1117/12.2508744

KEYWORDS: Time correlated photon counting, Luminescence, Fluorescence lifetime imaging, Diffusion, Rhodamine, Anisotropy, Microscopy, Time resolved spectroscopy, Sensors, Quantum efficiency, Calibration

Read Abstract +

Proceedings Article | 21 February 2019 Presentation + Paper

Fluorescence Recovery After Photobleaching (FRAP) with simultaneous Fluorescence Lifetime and time-resolved Fluorescence Anisotropy Imaging (FLIM and tr-FAIM)

Y. Teijeiro-Gonzalez, A. Le Marois, A. Economou, L. Hirvonen, J. Levitt, A. Beavil, R. Beavil, A. Crnjar, C. Molteni, E. Ortiz-Zapater, M. Parsons, K. Suhling

Proceedings Volume 10883, 108830A (2019) https://doi.org/10.1117/12.2508692

KEYWORDS: Anisotropy, Green fluorescent protein, Luminescence, Fluorescence lifetime imaging, Fluorescence anisotropy, Fluorescence resonance energy transfer, Diffusion, Sensors, Data modeling, Resolution enhancement technologies

Read Abstract +

Proceedings Article | 9 November 2016 Presentation

Spontaneous emission and non-radiative processes inside a hyperbolic metamaterial (Conference Presentation)

Diane Roth, Mazhar Nasir, Alexey Krasavin, Pavel Ginzburg, Wayne Dickson, Alix Le Marois, Klaus Suhling, David Richards, Viktor Podolskiy, Anatoly Zayats

Proceedings Volume 9920, 99201A (2016) https://doi.org/10.1117/12.2237735

KEYWORDS: Metamaterials, Electromagnetism, Energy transfer, Nanostructuring, Plasmonics, Dispersion, Gold, Nanorods, Pollution control, Current controlled current source

Read Abstract +

Proceedings Article | 26 July 2016 Presentation

Spontaneous emission and non-radiative processes inside a hyperbolic metamaterial (Conference Presentation)

Diane Roth, Mazhar Nasir, Wayne Dickson, Klaus Suhling, Pavel Ginzburg, David Richards, Anatoly Zayats

Proceedings Volume 9884, 98841D (2016) https://doi.org/10.1117/12.2227701

KEYWORDS: Metamaterials, Plasmonics, Fluorescence resonance energy transfer, Electromagnetism, Resonance energy transfer, Luminescence, Energy transfer, Molecules, Molecular energy transfer, Biosensing

Read Abstract +

Proceedings Article | 5 May 2016 Paper

Wide-field TCSPC-based fluorescence lifetime imaging (FLIM) microscopy

Klaus Suhling, Liisa Hirvonen, Wolfgang Becker, Stefan Smietana, Holger Netz, James Milnes, Thomas Conneely, Alix Le Marois, Ottmar Jagutzki

Proceedings Volume 9858, 98580J (2016) https://doi.org/10.1117/12.2227198

KEYWORDS: Single photon, Microscopy, Fluorescence lifetime imaging, Sensors, Luminescence, Cameras, Fluorescence lifetime imaging, Microscopy, Microchannel plates, Picosecond phenomena, Time correlated photon counting, Phosphorescence

Read Abstract +

SPIE Journal Paper | 3 September 2015 Open Access

Spectrally resolved fluorescence lifetime imaging of Nile red for measurements of intracellular polarity

James Levitt, Pei-Hua Chung, Klaus Suhling

JBO, Vol. 20, Issue 09, 096002, (September 2015) https://doi.org/10.1117/12.10.1117/1.JBO.20.9.096002

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Printed circuit board testing, Dielectrics, Data modeling, Confocal microscopy, Bioalcohols, Microscopy, Pollution control, Proteins

Read Abstract +

DOWNLOAD PAPER

Proceedings Article | 5 March 2015 Paper

Microsecond wide-field TCSPC microscopy based on an ultra-fast CMOS camera

Liisa Hirvonen, Zdenek Petrasek, Andrew Beeby, Klaus Suhling

Proceedings Volume 9329, 932939 (2015) https://doi.org/10.1117/12.2178567

KEYWORDS: Cameras, Image intensifiers, Image resolution, Ruthenium, Microscopy, Ultrafast phenomena, Data acquisition, Luminescence, CMOS cameras, Photon counting

Read Abstract +

Proceedings Article | 5 March 2015 Paper

Investigating cell membrane structure and dynamics with TCSPC-FLIM

Alix Le Marois, Dylan Owen, Klaus Suhling

Proceedings Volume 9329, 932938 (2015) https://doi.org/10.1117/12.2178563

KEYWORDS: Luminescence, Anisotropy, Fluorescence anisotropy, Molecules, Polarization, Motion models, Fluorescence lifetime imaging, Fluid dynamics, Environmental sensing, Single photon

Read Abstract +

Proceedings Article | 23 February 2011 Paper

Mapping intracellular viscosity by advanced fluorescence imaging of molecular rotors in living cells

Pei-Hua Chung, James Levitt, Marina Kuimova, Gokhan Yahioglu, Klaus Suhling

Proceedings Volume 7903, 790323 (2011) https://doi.org/10.1117/12.876257

KEYWORDS: Luminescence, Anisotropy, Fluorescence lifetime imaging, Absorption, Refractive index, Time resolved spectroscopy, Dielectrics, Quantum efficiency, Molecules, Picosecond phenomena

Read Abstract +

Proceedings Article | 11 February 2011 Paper

Simultaneous measurements of fluorescence lifetimes, anisotropy, and FRAP recovery curves

James Levitt, Pei-Hua Chung, Dominic Alibhai, Klaus Suhling

Proceedings Volume 7902, 79020Y (2011) https://doi.org/10.1117/12.875151

KEYWORDS: Luminescence, Anisotropy, Diffusion, Fluorescence lifetime imaging, Fluorescence anisotropy, Polarization, Green fluorescent protein, Proteins, Rhodamine, Time resolved spectroscopy

Read Abstract +

SPIE Journal Paper | 1 May 2008 Open Access

Effect of refractive index on the fluorescence lifetime of green fluorescent protein

Carolyn Tregidgo, James Levitt, Klaus Suhling

JBO, Vol. 13, Issue 03, 031218, (May 2008) https://doi.org/10.1117/12.10.1117/1.2937212

KEYWORDS: Luminescence, Refractive index, Green fluorescent protein, Proteins, Fluorescence lifetime imaging, Interfaces, Confocal microscopy, Glasses, Prisms, Molecules

Read Abstract +

DOWNLOAD PAPER

Proceedings Article | 5 March 2008 Paper

A high-content screening platform utilizing polarization anisotropy and FLIM microscopy

Daniel Matthews, Simon Ameer-Beg, Paul Barber, Glenn Pierce, Robert Newman, Boris Vojnovic, Leo Carlin, Melanie Keppler, Tony Ng, Klaus Suhling, Malcolm Irving

Proceedings Volume 6859, 685919 (2008) https://doi.org/10.1117/12.763310

KEYWORDS: Anisotropy, Fluorescence resonance energy transfer, Microscopy, Polarization, Luminescence, Fluorescence lifetime imaging, Proteins, Biosensors, Imaging systems, Microscopes

Read Abstract +

Proceedings Article | 15 February 2008 Paper

Multidimensional multiphoton fluorescence lifetime imaging of cells

James Levitt, Nicolas Sergent, Anne Chauveau, Marina Kuimova, Paul Barber, Daniel Davis, Klaus Suhling

Proceedings Volume 6860, 68601G (2008) https://doi.org/10.1117/12.763468

KEYWORDS: Luminescence, Anisotropy, Molecules, Fluorescence resonance energy transfer, Fluorescence lifetime imaging, Proteins, Fluorescence anisotropy, Sensors, Polarization, Confocal microscopy

Read Abstract +

Proceedings Article | 12 February 2008 Paper

Wide-field time-correlated single photon counting imaging for luminescence microscopy

Nicolas Sergent, James Levitt, Mark Green, Klaus Suhling

Proceedings Volume 6861, 68610K (2008) https://doi.org/10.1117/12.763561

KEYWORDS: Europium, Single photon, Cameras, Silver, Image intensifiers, Luminescence, Glasses, Sensors, Microscopy, Imaging systems

Read Abstract +

Proceedings Article | 15 October 2007 Paper

Wide-field photon counting imaging for fluorescence microscopy

Nicolas Sergent, James Levitt, Mark Green, Klaus Suhling

Proceedings Volume 6771, 67710X (2007) https://doi.org/10.1117/12.733793

KEYWORDS: Cameras, Single photon, Silver, Image intensifiers, Luminescence, Europium, Photon counting, Glasses, Ultrafast imaging, Nanocrystals

Read Abstract +

Proceedings Article | 15 October 2007 Paper

Time-resolved fluorescence microscopy

Klaus Suhling, Carolyn Tregidgo, Nicolas Sergent, James Levitt, Alex Pavlides, Mark Green, Graham Hungerford, Ana Rei, M. Isabel Ferreira

Proceedings Volume 6771, 677106 (2007) https://doi.org/10.1117/12.734097

KEYWORDS: Luminescence, Green fluorescent protein, Fluorescence lifetime imaging, Proteins, Photon counting, Time resolved spectroscopy, Refractive index, Anisotropy, Diffusion, Cameras

Read Abstract +

Proceedings Article | 10 February 2007 Paper

Refractive index sensing using fluorescence lifetime imaging (FLIM)

Carolyn Tregidgo, Klaus Suhling

Proceedings Volume 6442, 64420W (2007) https://doi.org/10.1117/12.699031

KEYWORDS: Refractive index, Green fluorescent protein, Fluorescence lifetime imaging, Luminescence, Interfaces, Glasses, Prisms, Proteins, Microscopes, Microscopy

Read Abstract +

Proceedings Article | 22 February 2006 Paper

Mapping the refractive index sensing range of the GFP fluorescence decay with FLIM

Carolyn Jones, Klaus Suhling

Proceedings Volume 6098, 609807 (2006) https://doi.org/10.1117/12.644732

KEYWORDS: Luminescence, Green fluorescent protein, Refractive index, Fluorescence lifetime imaging, Interfaces, Proteins, Environmental sensing, Mirrors, Molecules, Microscopy

Read Abstract +

Proceedings Article | 2 July 2001 Paper

Influence of the refractive index on EGFP fluorescence lifetimes in mixtures of water and glycerol

Klaus Suhling, Daniel Davis, Zdenek Petrasek, Jan Siegel, David Phillips

Proceedings Volume 4259, (2001) https://doi.org/10.1117/12.432486

KEYWORDS: Luminescence, Refractive index, Water, Absorption, Green fluorescent protein, Fluorescence lifetime imaging, Bacteria, Proteins, Quantum efficiency, Molecules

Read Abstract +

Proceedings Article | 7 May 1997 Paper

Fluorescence-lifetime imaging using a novel photon sensing module

David McLoskey, Klaus Suhling, David Birch

Proceedings Volume 2980, (1997) https://doi.org/10.1117/12.273544

KEYWORDS: Multiplexing, Sensors, Luminescence, Photodetectors, Detector arrays, Electronics, Picosecond phenomena, Single photon, Data acquisition, Microscopy

Read Abstract +

Proceedings Article | 1 April 1992 Paper

Fluorescence energy transfer to metal ions in lipid bilayers

David Birch, Klaus Suhling, A. Holmes, T. Salthammer, Robert Imhof

Proceedings Volume 1640, (1992) https://doi.org/10.1117/12.58267

KEYWORDS: Energy transfer, Luminescence, Ions, Cobalt, Carbon dioxide, Molecules, Laser spectroscopy, Biochemistry, 3D modeling, Metals

Read Abstract +

Proceedings Article | 1 May 1990 Paper

Array fluorometry: the theory of the statistical multiplexing of single-photon timing

David Birch, Klaus Suhling, Sheila Smith, A. Dutch, Robert Imhof

Proceedings Volume 1204, (1990) https://doi.org/10.1117/12.17683

KEYWORDS: Single photon, Multiplexing, Luminescence, Biochemistry, Laser spectroscopy, Statistical multiplexing, Data conversion, Radon, Detector arrays, Photomultipliers

Read Abstract +