Dr. Kotaro Kai Profile

Dr. Kotaro Kai

at Tokyo Women's Medical Univ

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Proceedings Article | 13 February 2008 Paper

In vivo cell kinetics of the bone marrow transplantation using dual colored transgenic rat system

Kotaro Kai, Satoshi Teraoka, Yasushi Adachi, Susumu Ikehara, Takashi Murakami, Eiji Kobayashi

Proceedings Volume 6868, 68680R (2008) https://doi.org/10.1117/12.762577

KEYWORDS: Bone, In vivo imaging, Luminescence, Green fluorescent protein, Transplantation, Blood, Imaging systems, Stem cells, Visualization, Animal model studies

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Because bone marrow is an adequate site for bone marrow stem cells, intra-bone marrow - bone marrow transplantation (IBM-BMT) is an efficient strategy for bone marrow transplantation (BMT). However, the fate of the transplanted cells remains unclear. Herein, we established a dual-colored transgenic rat system utilizing green fluorescent protein (GFP) and a luciferase (luc) marker. We then utilized this system to investigate the in vivo kinetics of transplanted bone marrow cells (BMCs) after authentic intravenous (IV)-BMT or IBM-BMT. The in vivo fate of the transplanted cells was tracked using an in vivo luminescent imaging technique; alterations in peripheral blood chimerism were also followed using flow cytometry. IBM-BMT and IV-BMT were performed using syngeneic and allogeneic rat combinations. While no difference in the proliferation pattern was observed between the two treatment groups at 7 days after BMT, different distribution patterns were clearly observed during the early phase. In the IBM-BMT-treated rats, the transplanted BMCs were engrafted immediately at the site of the injected bone marrow and expanded more rapidly than in the IV-BMT-treated rats during this phase. Graft-versus-host disease was also visualized. Our bio-imaging system using dual-colored transgenic rats is a powerful tool for performing quantitative and morphological assessments in vivo.

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