Mr. Krzysztof Szulzycki Profile

Krzysztof Szulzycki

at Nicolaus Copernicus University

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 14 March 2018 Presentation

High-speed remote focusing for improvement of 3D imaging performance in confocal microscopy and optical coherence microscopy (Conference Presentation)

Krzysztof Szulzycki, Viktoriya Savaryn, Ireneusz Grulkowski

Proceedings Volume 10499, 1049910 (2018) https://doi.org/10.1117/12.2290074

KEYWORDS: Confocal microscopy, Bessel beams, Curium, Optical coherence microscopy, Image acquisition, Image quality, Point spread functions, Microscopy, Stereoscopy, 3D acquisition

Read Abstract +

Confocal Microscopy (CM) enables 3-D high-resolution images acquisition of biological samples by confocal pinhole application. Whereas transverse scanning can be provided by implementation of scanning mirrors, axial scanning can be achieved by moving the sample, which limits the acquisition speed. On the other hand, with coherence gating Optical Coherence Microscopy (OCM) allows to generate micrometer resolution, cross-sectional images and volumetric data on the internal structure of back-scattering objects. The aim of our study was to assess imaging performance of different designs of CM and OCM with tunable lens technology. Mechanical scanning in axial direction was replaced by remote control of the objective focus position with the two types of lenses: Electrically Tunable Lens (ETL; up to 500 Hz) and Acousto-Optic Lens (AOL; 250 kHz). We compared focus tuning ability of ETL and AOL technologies by applying beam profiling and wavefront sensing. We assessed the impact of tunable lens technology on the CM and OCM system performance. We designed the confocal microscopic system with Bessel beam illumination to extend the depth-of-focus. The axial scanning of the collection point will be provided by our AOL. Combination of Bessel beam illumination and AOL allowed high-speed image acquisition from well-controlled depth positions along with better quality of PSF due to self-healing properties of the Bessel beam. We compared image quality of the proposed configurations with the standard design using biological specimens. To conclude, tunable lens technology implemented to CM and OCM instruments enables enhancement of instrument performance.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years