DNA hybridization can be measured with enhanced sensitivity based on localized surface plasmon (LSP) induced by
surface nanowire structure. Changes made to the structure result in higher plasmon momentum, which can be coupled to
a particle plasmon induced by gold nanoparticles to which DNA molecules are adsorbed. With the insight gained from
near-field pattern via calculation, target localization effect is also experimentally shown. We expect that orders of
magnitude can be improved in terms of sensitivity if one is to combine the effect of particle-to-LSP coupling and target
localization scheme.
In this study, we explore the relation between near-field and far-field characteristics of plasmon-enhanced total internal
reflection fluorescence (TIRF) imaging. It was found that a significant disparity exists between near-field intensity
maximum of evanescent fields and reflectance minimum in the far-field. The disparity tends to be larger when plasmons
are localized by surface nanostructures. Experimental data with nanogratings and nanoislands as well as theoretical
results are presented. The disparity can be considered to optimize plasmon-enhanced TIRF microscopy.
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