To enhance discrimination of UV-laser-induced-fluorescence based bio-aerosol-detection-system, a UV-laser is described that allows multiple wavelength excitation of bio-aerosols and both fluorescence spectral and time-decay analysis. The latter requiring sub-ns pulse duration, a two-stage-amplifier boosts a 20-µJ-1064-nm-500-ps-actively-Q-Switch microchip-oscillator output energy up to 2.5 mJ. After frequency doubling and beam splitting, 20-µJ-293-and-337-nm pulses are generated by two different periodically-poled-KTP (parametric generation) and BBO (frequency doubling) crystal arrangements. In order to get distinct fluorescence signals for each wavelength, the beams are then time-delayed with two optical fibers of different lengths and launched into a chamber for bio-aerosol excitation connected to a fast detection system.
Laser Induced Fluorescence (LIF) could permit fast early warning systems either for point or stand-off detection if a reliable classification of warfare biological agents versus biological or non-biological fluorescing background can be achieved. In order to improve LIF discrimination capability, a new system is described in which the fluorescence pattern is enriched by the use of multiple wavelength delayed excitation while usual spectral fluorescence analysis is extended to time domain to use both aspects as criteria for classification. General considerations and guidelines for the system design are given as well as results showing good discrimination between background and simulants.