We combine pulsed laser, supercontinuum radiation source and fast single-photon counting peripherals to obtain a multifunctional
micro/nano-scope. This provides us with better spatial and temporal resolution to observe fast dynamics.
Performing fluorescence correlation spectroscopy for fast dynamics (<μs) with sub-diffraction limit resolution to observe
the initial state of single-lipid dynamics in supported lipid bilayers and living cells is our goal. Lipid raft serves as a
platform for recruiting signaling components of effective signal transduction. However, the dynamics of sub-200nm
rapidly aggregated lipid rafts are still not elucidated in living cells. We here report our recent progress on the
construction of this multi-functional stage-scanning fluorescence micro/nanoscope for single-lipid dynamics study.