The 3D image sensor simulating human vision has an important application prospect in stereo camera,human-computer interaction,and three-dimensional measurement. Based on the optical properties of 3D vision sensor,the character of TFT LCD’s high precision made pixel and high flatness are used to make color image target,and this operation overcomes the limitation of the static target. The optimization method ofHarris conner detection algorithm is also discussed in this paper,then a new measuring method of 3D sensor color distortion is proposed. The calibration,which calibrate the internal and external parameters of 3Dvision sensor and color distortion,is completed by the programming software of 3D sensor calibration. The experimental results demonstrate the proposed calibration method is feasible.