The objective of this work was to attain essential parameters by using a Gompertz model that employed a new approach of wavelength modulation spectroscopy (WMS) to describe the microorganism growth. The measurement method of WMS introduces noninvasive technique instead of complicated invasive microorganism operation analysis and quickly obtains the accurate real-time measurement results. By using the WMS measurement, the specific growth curve of microorganism growth clearly displayed every three minute, which has characteristics of high sensitivity, high spectral resolution, fast time response and overcomes the randomness and error operation of traditional analysis methods. The measurement value of BF and AF in the range of 1.008 to 1.043 and the lower MSE showed that Gompertz model can fit the data well and be capable of describing bacteria growth rate and lag time. The results of experiment data suggested that the specific growth rate of microorganism depends on the temperature. With the increase of temperature ranging from 25 °C to 42 °C , the lag time of bacteria growth has been shortened. And the suitable temperature of bacteria growth is about 37 °C . Judging from the growth rate of microorganisms, we can identify the microbial species, not only to improve the precision and efficiency, but also to provides a rapidly sensitive way for microbial detection. The lag time of microorganism growth also provides a great application prospect for shelf life of the food safety.
High-resolution absorption measurements of H<sub>2</sub>O using direct absorption spectroscopy technology
were made to acquire the spectroscopic parameters of High Resolution Transmission Molecular
Absorption Database (HITRAN database). The most important spectral parameters frequently used in
the researchers and study, was calculated from the integrated absorbance area of H<sub>2</sub>O at the different
temperature from 296K to 923K of the spectrum near 1397nm based on the distributed feedback laser.
From the result of experiment, the calculated spectral parameters was compared with the literature
values from HITRAN database. The method and results of the measurement obtained from this paper
are expected to be helpful in the supply of the sufficient accuracy spectral parameters in the practical