A currently available 2-D high-resolution, optical molecular imaging system was modified by the addition of a
structured illumination source, Optigrid<sup>TM</sup>, to investigate the feasibility of providing depth resolution along the
optical axis. The modification involved the insertion of the Optigrid<sup>TM</sup> and a lens in the path between the light source
and the image plane, as well as control and signal processing software. Projection of the Optigrid<sup>TM</sup> onto the imaging
surface at an angle, was resolved applying the Scheimpflug principle. The illumination system implements
modulation of the light source and provides a framework for capturing depth resolved mages.
The system is capable of in-focus projection of the Optigrid<sup>TM</sup> at different spatial frequencies, and supports the use
of different lenses. A calibration process was developed for the system to achieve consistent phase shifts of the
Optigrid<sup>TM</sup>. Post-processing extracted depth information using depth modulation analysis using a phantom block
with fluorescent sheets at different depths.
An important aspect of this effort was that it was carried out by a multidisciplinary team of engineering and science
students as part of a capstone senior design program. The disciplines represented are mechanical engineering,
electrical engineering and imaging science. The project was sponsored by a financial grant from New York State
with equipment support from two industrial concerns. The students were provided with a basic imaging concept and
charged with developing, implementing, testing and validating a feasible proof-of-concept prototype system that was
returned to the originator of the concept for further evaluation and characterization.