Heating has been known to cure cancer for over 2000 years1, and recent studies have confirmed this in the treatment of basal cell carcinomas (BCC)2. The application of uncontrolled heating often results in unacceptable scarring. Our ability to control the delivery of heat using a forward-looking infrared (FLIR) camera and a modulated long pulsed (LP) Nd:YAG 1064nm laser has resulted in better outcomes in comparison to traditional destruction techniques3. Use of optical coherence tomography (OCT) enhances our ability to define the tumor margins prior to treatment. The combination of FLIR and OCT allows very precise treatment of BCCs with at least comparable outcomes to conventional LP Nd:YAG treatments.
KEYWORDS: Skin, In vivo imaging, Associative arrays, Tumors, Melanoma, Image resolution, Diagnostics, Denoising, Convolutional neural networks, Chemical elements
Multiphoton microscopy can provide sub-micron resolution images of living tissues in their
native environment with chemical contrast. We recently reported on a fast large area multiphoton
exoscope (FLAME) for rapidly mapping out macroscopic tissue areas (cm-scale) with microscopic
resolution. In this presentation we demonstrate the imaging capability and the clinical utility of this
system by performing a pilot study on ex vivo imaging of benign and malignant pigmented lesions of
human skin. We identify morphological features such as cytological atypia, lentiginous hyperplasia,
migration of melanocytes and demonstrate the value of sampling large tissue volumes for capturing the
lesion heterogeneity.
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