The study of biological objects using a light microscope is one of the main methods of diagnostic practice in many areas of natural science. Most modern methods of researching objects using a light microscope include preliminary preparation of objects. However, in this case it is impossible to achieve a high degree of reliability of information about the object under study.
The aspects of the use of dioptric optics for DUV microscopy are considered. The transition to the NUV and DUV range can significantly improve the resolving power of the microscope. Different kinds of crystals can be used as optical materials.
Some aspects of the expansion of the spectral range for lens objectives for microscope are considered. It is proposed to use two types of optics. The first type provides operation in the near ultraviolet range (NUV) plus a visible range. The second type provides operation in the visible and near infrared (NIR) spectral range.
The results of optical designs of objectives for budget light microscopes are presented, including for providing special methods of contrasting. For the design, original optical constructions are applied.
The main difference is a significant expansion of the linear field, which reaches 50 mm in the image plane. However, in this case, a planapochromatic correction must be achieved for the usual visible spectral range. Such a parameter of objectives, like over large working distances – is an additional advantage.