A characteristic feature of diabetes mellitus is an increase in blood glucose levels and the development of hyperglycemia, which provokes the development of metabolic changes at the level of cells and tissues of the body. The dysfunction of pancreatic beta cells with impaired insulin secretion and a decrease in their mass is the main sign of the development of both types of diabetes. Thus, beta cells are the main target in the development of new therapeutic approaches.
In this work, using a model of pancreatic beta cells (RINm5F), the effect of photosensitizer-free laser-induced singlet oxygen (SO) on the bioenergetics of this type of cells was studied. It was found that laser exposure affects a number of parameters characterizing the bioenergetics of cells: mitochondrial membrane potential, NADH, FAD and ATP levels. The totality of the results obtained may indicate the potential possibility of using laser-induced SO in the regulation of beta cell functions.
This study contains analysis of existing approaches for detecting photosensitizer-free laser-induced singlet oxygen, including their ability to quantitatively measure the relationship between a dose of laser radiation and the amount of singlet oxygen produced.
An approach to visualization of the vascular bed with the possibility of assessing changes in blood filling and identifying diagnostically significant periodic changes in the signal by analyzing speckle images is proposed. The effect of singlet oxygen by direct excitation of an oxygen molecule by 1267 nm laser radiation on changes in the vascular bed parameters was studied using this approach.
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