Many chemical sensors based on fluorescence spectroscopy have been reported in applications, ranging from biomedical
and environmental monitoring to industrial process control. In these diverse applications, the analyte can be probed
directly, by measuring its intrinsic absorption, or by incorporating some transduction mechanism such as reagent
chemistry to enhance sensitivity and selectivity. Hydrogen Peroxide is a colorless liquid. It is a common oxidizing and
bleaching agent. It plays an important role in High Power Laser such as Chemical Oxygen Iodine Laser (COIL). As it is
on the Hazardous substance list and on the special health hazard substance list, detection of Hydrogen Peroxide is of
great importance. In the present study the detection of hydrogen Peroxide is by fluorescence quenching of laser grade
dye Rhodamine B. Estimation of rate constant of the bimolecular quenching reaction is made.
The development of sensors based on immobilized fluorescent reagent is a matter of growing interest. Chemiluminescence seems to be attractive because light is generated through the chemical reactions. No light source is needed, which makes the experimental set up very simple. In present work, the sensor presented is an optical sensor based on fluorescence quenching. Fluorescence quenching refers to any process, which decreases the fluorescence intensity of a certain fluorophore. Acetone is a commercially used solvent of great importance as it has got wide chemical and biomedical applications. It is on the hazardous substance list as well as on the special health hazard substance list. Hence identification of acetone has an immense importance. Fluorescence quenching of 7-Diethylamino-4-trifluoro methyl Coumarin is reported here. It was found that the quenching observed was of dynamic in nature. It was also observed that quenching of the fluorescence of the indicator had a full reversibility. As it has a full reversibility, an optical sensor for acetone can be constructed on this quenching.