We present a deformable mirror based remote focusing method for three-dimensional imaging in high-resolution microscopy systems. The method relies on predefined mirror mode arrays that are obtained during initial mirror training step with a low complexity wavefront-sensing module. The imaging plane can be refocused over distances over a hundred times greater than the original depth of field of the objective lens along the optical axis at millisecond rates. We will demonstrate the combination of the remote focusing method with spatiotemporally focused two-photon excitation applied to three-dimensional imaging of biological samples.
We present a novel swept source optical coherence tomography configuration, equipped with acousto-optic deflectors
that can be used to simultaneously acquire multiple B-scans OCT images originating from different depths. The
sensitivity range of the configuration is evaluated while acquiring five simultaneous B-scans. Then the configuration is
employed to demonstrate long range B-scan imaging by combining two simultaneous B-scans from a mouse head
We present a time-domain polarization-sensitive (PS) optical coherence tomography configuration operating at 830 nm, equipped with multichannel acousto-optic deflectors and single photodetectors. The system is used to simultaneously acquire interference information from multiple PS channels and to enable measurement and imaging of backscattered intensity to create both PS and polarization insensitive images. Our approach enables multiple channel imaging without need to divide the object signal. Here, we employ our system in order to demonstrate PS imaging of a thermally damaged muscle tissue.
In this paper we present a frequency multiplexed optical coherence tomography imaging method in which simultaneous
data acquisition is enabled by a multiplexer. The multiplexer is based on a pair of Bragg cells, simultaneously driven at
several frequencies that enable multiple paths in the reference arm. Multiple beams are created in the multiplexer and
then recombined again so that the resulting beam multiplex can be inserted into a single mode fiber. In this paper we
discuss the design principles for a multiplexer that supports the reflectivity measurements as well as polarization
sensitive imaging at several different depths. We show how to design a system with a low footprint, while allowing
broad optical bandwidth to be used for imaging in multiple channels with little crosstalk between adjacent channels.
We present a novel time-domain polarization sensitive optical coherence tomography configuration operating at 830 nm,
equipped with multichannel acousto-optic deflectors (AOD)s. The system can be used to simultaneously acquire
interference information from multiple polarization-sensitive channels and to enable measurement and imaging of
backscattered intensity, birefringence, and fast optic axis orientation. The system is employed here to demonstrate
polarization sensitive imaging of a thermally damaged muscle tissue.