A microfluidic system was developed and combined with optical tweezers for single cell sorting. This system consists of
a glass chip of 300 μm thickness with an etched crosswise channel structure, a silicon layer for sealing and a PMMA
substrate for tubular coupling. Selected cells are trapped and moved in perpendicular direction to the main flow for
recovery in special reservoirs and further evaluation (e.g. by polymerase chain reaction, PCR). In addition, maximum
light doses and exposure times for maintaining cell viability were determined.