This study aims towards applying the intrinsic polarized fluorescence technique for obtaining valuable
information from human cervical tissue samples. The efficacy of this technique is tested in human tissues by comparing
its diagnostic capabilities with the bulk fluorescence. It is seen that biochemical information is hidden due to the
presence of distortions by tissue scattering and absorption in the fluorescence spectra. Intrinsic tissue fluorescence
provides a complete understanding of the biochemical and/or morphological changes that take place during the
progression of disease. Recording of the experimental data and thereafter subsequent extraction of the intrinsic
fluorescence serves as fingerprints towards determining the occurrence of these diseases.
Here we report a comparative study of intrinsic versus bulk polarized fluorescence in cervical tissues. Intrinsic
fluorescence is seen to be a more sensitive technique than bulk fluorescence for diagnosis of cervical cancers. Attempts
have been made to study the changes in the amount of different fluorophores found in the epithelial and stromal layer of
cervical tissue (both normal and cancerous). It has been seen that collagen decreases and NADH increases as a healthy
cervical tissue develops into a cancerous one. Intrinsic fluorescence provides more consistent discriminating results as
compared to bulk polarized fluorescence. It is also more sensitive in giving biochemical information from the different
layers in cervical tissue. It may be concluded that intrinsic fluorescence shows promise as a viable tool for providing
valuable insights towards fruitful diagnosis of the various stages of disease development and changes occurring with age.