KEYWORDS: Photons, Quantum emitters, Pulsed laser operation, Point spread functions, Super resolution, Semiconductors, Photoluminescence, Super resolution microscopy, Nanostructures, Time correlated single photon counting
A time-dependent likelihood distribution for analyzing time correlated single photon counting data from a four-pixel time-resolved single molecule localization microscopy experiment is discussed. It is generated by accounting for the probabilities to record photons from two emitters, background counts, and dark counts during two different time channels relative to each incident laser pulse in the experiment. Maximizing the distribution enables localization of each emitter in a dual emitting nanostructure based on the disparate photoluminescence lifetimes of the emitters, even when both emitters are simultaneously in an emissive state. The technique is demonstrated using simulated photon counting data from a hypothetical non-blinking dual-emitter nanostructure in which the distance between the two emitters is less than 10-nm.
A dual-color super-resolution microscope with polarization and orientation-resolving capabilities is presented. Combining single-molecule localization methods with simultaneous polarization measurements enables the determination of the orientation of single emitters, such as quantum dot nanocrystals, with sub-10 nm precision. Additional simultaneous spectral characterization of particle emission allows the capture of multiple optical properties that impact energy transfer. We report on the instrumentation development and the results from coupled quantum dot clusters.
A hybrid Fourier transform infrared (FTIR) / quantum cascade laser (QCL) spectrometer is introduced for the analysis of gas-phase chemical kinetics, including the study of alkyl halide photolysis reactions. The FTIR provides broadband spectral survey information and the QCL laser system provides improved detection limits and acquisition speeds, albeit over limited wavelength domains. A kinetic model for the photolysis of methyl iodide is introduced which suggests that both the steady state products, such as methanol, and transient intermediates may be monitored using the hybrid setup. Preliminary results use an external cavity QCL to rapidly measure the spectrum of methanol from 2200-1960 cm-1 in ~2 seconds, which is sufficiently fast to capture the chemical dynamics predicted by the model to occur during the first several seconds of photolysis.
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