In this work, we demonstrate the ability to image and quantify airway changes, we were able to quantify a decrease in airway compliance. The proposed approach will enable further investigations of using OCT assessing pulmonary injury to prevent/treat ARDS using a chlorine inhalation injury model, as well as diagnosing of large airway injury and compliance change due to airway toxic chemical exposure. With enhanced portability over conventional bronchoscopy, we believe our system is capable of field hospital deployment and investigating airway conditions in warfighters. Combining OCT and pressure transducer with bronchoscopy would enhance assessment and treatment of large airway chemical injury.
In this work, we demonstrate the ability to image and quantify airway changes, edema, and epithelial layer separation using OCT and automated tissue boundary identification in the rabbit large airways as early as 30-minutes post-chlorine gas exposure. We propose this novel approach will enable further investigations into using OCT for pre-hospital and point-of-care diagnostics of large airway injury due to airway toxic chemical exposure. With enhanced portability over conventional bronchoscopy, we believe our system is capable of field hospital deployment and investigating airway conditions in warfighters. Combining OCT with bronchoscopy would enhance the assessment and treatment of large airway chemical injury.
Spectrally encoded interferometric microscopy (SEIM) is capable of detecting nanometer displacement at a frame rate in the kilohertz regime. By employing a wavelength-sweeping laser and a spectral disperser, SEIM can achieve en face imaging via one-axis scanning. In this study, we compared different processing algorithms for visualizing cilia-induced motion. Our Doppler-based method, combined with phase stabilization and bulk motion correction, provides the highest sensitivity for measuring ciliary beating frequency amongst the tested methods. Traveling waves induced by coordinated cilia motion were visualized. These results demonstrate the potential clinical utility of SEIM for monitoring respiratory function and therapeutic effects.
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