Amanita is one of the most well known basidiomycetes genus throughout the world because some of its species
that are acknowledged due to their toxic and/or hallucinogenic properties. Considering these properties in the last
decades become more important for scientist to dignify exactly the chemical content of these mushroom species. Latter
researches shown that A. phalloides contain two main groups of toxins: the amatoxins and the phallotoxins. As regards
A. rubescens there are not so much studies referring to its biochemical "fingerprint".
Two species (A. rubescens and A. phalloides) of Amanita genus were studied in order to determine the
biochemical hall-mark at nanoscale for these basidiomycete's species. Parts as caps, gills, flesh and stem of these
mushrooms were analyzed on quadrupole mass spectrometer engaged with a gas chromatograph (GC-qMS) using
selective ion monitoring mode (SIM). The biochemical profiles of these species had shown the presence of compounds
like fatty acid methyl esters (FAMEs), alkaloids, and volatile compounds (including alcohol compounds, carbonyl
compounds, terpenes). The levels of biochemical compounds from these species were compared between the two types
of species and also between young, mature and old samples for the same species as well as between the parts of
mushroom.
After this comparison were between the two species it was observed that in case of A. phalloides the alkaloid
content were higher usually with almost 50 %. As regards presence of volatile compounds they have almost similar level
in both mushroom species. Considering the levels of fatty acid methyl esters, their levels were higher with 30 - 40 % in
case of A. rubescens.
Biomonitoring studies for estimation population health surveillance and exposure risk assessment of different
chemical contaminants such as chlorinated compounds or polycyclic aromatic hydrocarbon (PAH) compounds has
become an important task especially after the Stockholm Convention. If before the toxicological evaluation of humans
were done using invasive methods like surgery or pricking, today many scientists tried to elaborate non-invasive
analytical methods without disparage the final results.
During the last years studies it was observed a relative higher pollution with organochlorine and polycyclic
aromatic hydrocarbon compounds in surrounding regions of Dej, Transylvania. These past studies shown that pollution
with chlorinated compounds as chlorinated solvents are attributed to the industrial activities from this region. The levels
in soil and river water of these compounds were: ≈ 20 - 60 μg·kg-1 and ≈ 15 - 45 μg·L-1, respectively. In case of PAHs
the following results were obtained for soil and river water: for two ring specie was between ≈ 26 - 35 μg·kg-1 and ≈ 21
- 30 μg·L-1, respectively; for three ring species was 15 - 35 μg·kg-1 and ≈ 10 - 24 μg·L-1, respectively; and in case of
four ring species was between 10 - 20 μg·kg-1 and ≈ 3 - 15 μg·L-1, respectively.
These results carry on concern regarding the bioaccumulation of these pollutants by humans through food web
chain. In order to establish the uptake level of these compounds by humans, home grown animal hair as pig and cow
were analyzed through SIM-GC-MS mode and ECD-FID-GC. The presence of chlorinated solvents detected in pig and
cows hair were as follows: compounds from chloromethane family ≈ 5 - 10 ng·kg-1 dry weight; compounds from
chloroethane family ≈ 7 - 34 ng·kg-1 dry weight for pig hair, and ≈ 12 - 17 ng·kg-1 dry weight for compounds from
chloromethane family and 14 - 48 ng·kg-1 dry weight for cow hair. Difference between accumulation levels of PAH
metabolites were observed also between cow and pig hair samples.
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