In this paper, a novel fragile image watermarking scheme based on singular value decomposition (SVD) using genetic algorithm (GA) is proposed. Every line of watermark is scaled by using multiple scaling factors (SFs). Host image is divided into blocks. Watermarked image is obtained by embedding a different line of the watermark to singular values (SVs) of the every block. In this proposed method, the SFs are optimized using GA to obtain maximum transparency. Experimental results indicate that the method reached the highest possible transparency. Fragility of the watermark under various attacks such as rotating, rescaling and sharpening is tested. When an attack does not occur, exactly the original extracted watermark is obtained; on the other hand, the extracted watermark is intensely distorted.