As an alternative imaging technique to conventional IR microscopy, a mid-infrared photothermal microscopy has been suggested to achieve spatial resolution at the submicrometer level and the inherent chemical contrast upon vibrational excitation. It also has substantial potential for real-time imaging of live organisms to observe the cellular dynamics without photodamage or photobleaching of fluorescent labels. We performed real-time imaging of oligodendrocytes to investigate cellular dynamics and observed a photothermal contrast associated with traveling protein complexes on an axon of live neutrons. We anticipate that mid-infrared photothermal imaging will be of great use for gaining insights into the field of biophysical science, especially with regard to cellular dynamics and functions.
Conference Committee Involvement (2)
Advanced Chemical Microscopy for Life Science and Translational Medicine 2021
6 March 2021 | San Francisco, California, United States
Advanced Chemical Microscopy for Life Science and Translational Medicine
1 February 2020 | San Francisco, California, United States