The world-wide explosion of commercial microComputed Tomography (microCT) system emplacement has led to dayin,
day-out access to laboratory scanners. Most biologically-oriented microCT facilities must characterize large numbers
of samples rapidly at moderate spatial resolution (e.g., 10-20 μm isotropic volume elements, voxels). Scanning multiple
specimens simultaneously is one efficient solution. Sample positioning is critical if the region of interest of each
specimen is to be imaged without increasing the number of slices recorded (i.e., data acquisition and reconstruction
times). Three very different, multiple sample data acquisitions are reported: mouse heart tissue calcification, rat spinal
fusion and mouse tibial bone cancer models
The microstructure of pathological biomineral deposits has received relatively little attention, perhaps, in part because of the difficulty preparing samples for microscopy. MicroCT avoids these difficulties, and laboratory microCT results are reviewed for aortic valve calcification (human as well as a rabbit model), for human renal calculi (stones) and for calcinoses formed in juvenile dermatomyositis (JDM). In calcified aortic valves of rabbits, numerical analysis of the data shows statistically significant correlation with diet. In a large kidney stone the pattern of mineralization is clearly revealed and may provide a temporal blueprint for stone growth. In JDM calcified deposits, very different microstructures are observed and may be related to processes unique to this disease.