We have experimentally shown that selective modification of the PMMA surface with a femtosecond laser irradiation could control the adhesion of cells to the PMMA surface. Using a femtosecond laser, periodic nanostructures were formed indirectly on a part of the PMMA film surface. By performing a cell cultivation test, the cell adhesion density to the substrate was measured. As a result, the cell adhesion density was 7 times higher in the laser processed area than in the non-processed area. This is considered to be due to the fact that the cell adhesive protein adheres well to the surface processed by the femtosecond laser.