The results of studies of exosome visualization obtained using multiphoton fluorescence spectroscopy for healthy volunteers and patients with colorectal cancer are presented. Standard centrifuging technology was used to isolate exosomes from blood plasma. Exosome samples were investigated using the femtosecond laser pumping at the wavelength of 760 nm with and without FITC (Fluorescein isothiocyanate) labels. In particular, the exosomes with CD9 surface glycoprotein of the tetraspanin family was shown to be studied without labeling using FLIM (fluorescent lifetime microscopy).
The study of exosomes of saliva and blood plasma by THz laser spectroscopy was carried out. Exosomes were sampled from patients with colorectal cancer (n = 6) and healthy volunteers (n = 5). A substantive examination of the samples absorption spectra was performed using the method of canonical correlation analysis. The presence of Glycine, LAlanine, Mannose was revealed everywhere. The Mannose content was less in exosomes samples corresponding to colorectal cancer in comparison with exosomes samples from healthy volunteers.