Light sheet fluorescence microscopy (LSFM) enables fast 3D imaging of live cells, however the traditional LSFM geometry is not compatible with conventional multiwell plates used in high content microscopy.
We have developed an automated LSFM platereader based on an oblique plane microscope (OPM) that is compatible with multiwell plates. The system enables automated studies of cells cultured in a collagen matrix. Cells across 30 different conditions are imaged every 5 minutes for 12 hours. A custom 3D segmentation and tracking pipeline analyses cell morphological dynamics, allowing the study of a range of treatment conditions on the ability of cancer cells to change shape and invade.
Aggregation of misfolded proteins is a characteristic hallmark of many neurodegenerative disorders, such as Parkinson’s, Alzheimer’s and Huntington’s diseases. The ability to observe these aggregation processes and the corresponding structures formed in vitro or in situ is therefore a key requirement to understand the molecular mechanisms of these diseases. We report here on the implementation and application of Stimulated Emission Depletion (STED) microscopy to visualize the formation of amyloid fibrils in vitro.