The effect of PDT on ATP level in the Leukemic monocyte lymphoma cell line (U937) was studied using two different
photosensitizers, PpIX and Hypericin. U-937 cells were irradiated (with and without ALA) and stored at room
temperature. An LED lamp with a maximum peak of 637nm was used for ALA-PDT as a light source. A Na-Li lamp
with a wavelength region of 560-730nm was used for Hypericin-PDT as a light source. We measured cell viability, the
number of viable cells and ATP luminescence. As a result, it is evident that the irradiation time in the most effective PDT
is equal to the time of the highest ATP level in the cells in ALA-PDT. This study suggests that it is possible to obtain
suitable PDT conditions inducing apoptosis by measuring the ATP level in ALA-PDT. On the other hand, ATP level in
Hypericin-PDT was increased with the decreasing in the cell viability. Hypericin-PDT induced apoptosis just after
irradiation.
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