We describe the concept of laser rastering flow cytometry, where a rapidly scanning laser beam allows counting and
classification of cells at much higher rates than currently possible. Modifications to existing flow cytometers to
implement the concept include an acousto-optic deflector, fast analog-to-digital conversion, and a two-step
digital-signal-processing scheme that handles the high data rates and provides key assay information. Results are shown that
prove the concept, demonstrating the ability to resolve closely spaced cells and to measure cells at rates more than an
order of magnitude faster than on conventional flow-cytometer-based hematology analyzers.