In this work, the digital holography technique was used to examine different types of colors on the painting. Quantitative phase imaging by in-line digital holography is used to get the phase profile of image bearing beam from total internal reflection, which is modulated by the paintings of different kinds of color on the surface of internal reflection. The experimental results were shown that the reconstructed images of different kinds of colors which were painted on both black and white fine face papers could be observed more details than those on rough face papers. The reconstructed images have been shown the details of homogeneity of different kinds of colors. By improving the technique, this approach would be used for the examination of particular piece of paintings in the future.