Methods of two-photon microscopy are widely used in the study of biological objects, in particular, skin, due to the possibility to study objects both on the surface and at depth without attracting additional fluorophores due to endogenous autofluorescence. In this paper, the methods of image analysis of the AF signal and SHG signal are applied to assess the condition of the skin during the development of lymphedema. It is shown that for groups of healthy tissue and lymphedematous using SAAID distribution histograms, changes in tissues can be detected.
A model of lymphedema was developed using laboratory animals. During lymphatic edema enhancement, a study was made of changes in the structure of biological tissues by interference optical methods in the near infrared region of the spectrum. Statistical methods were applied for describing textures of the experimental images of the affected tissues, informative features of the dynamics of fibrosis development were identified.