In vivo optical trapping is a novel applied direction of an optical manipulation, which enables one to noninvasive measurement of mechanical properties of cells and tissues in living animals directly. But an application area of this direction is limited because strong scattering of many biological tissues. An optical clearing enables one to decrease the scattering and therefore increase a depth of light penetration, decrease a distortion of light beam, improve a resolution in imaging applications. Now novel methods had appeared for a measurement an optical clearing degree at a cellular level. But these methods aren’t applicable in vivo. In this paper we present novel measurement method of estimate of the optical clearing, which are based on a measurement of optical trap stiffness. Our method may be applicable in vivo.
In this paper, we present a novel simple technique of Fourier-transform holographic microscopy (FTHM). Simplicity of the scheme, possibility to use a small image sensor and provide compensation of aberration, enable one to construct inexpensive holographic microscopes. We experimentally compare FTHM with in-line holographic microscopy. In this paper, we present experimental scheme of FTHM, description of used algorithms and experimental results for an amplitude test object and biological samples (blood smears).
We present a novel technique for 3D-tracking micro- and nanoparticles with original lens-free dark-field holographic microscope. Combining lens-free and dark-field microscopy principles this technique allows for high accuracy localization of micro- and nanoparticles using single hologram acquired with compact setup built with minimal use of optical components. In this paper, we present technique of particles localization, experimental setup, technique of digital correction of spherical aberration, results of simulation and experimental data.