The rezazurin assay for screening of bacterial growth remains the most popular method for express diagnostic tests. It is based on the change of colour and uorescence properties of the respective dye due to bacterial breathing but its conventional quantitative practical implementation requires a relative expensive and cumbersome spectrophotometric equipment. As an alternative approach, we propose to shift a focus from hardware to software. Preliminary results announced in this work show that the processing RGB colour channels for simple photographic images of resazurin assay plates with mycobacterium tuberculosis provides an opportunity to reconstruct the target growth curve with an accuracy comparable with results obtained using the conventional photometric uorescence curves.
We have developed a methodology for the study of deactivated strains of Mycobacterium tuberculosis. Strains of the Beijing species obtained from pulmonary patient secrete (XDR strain) and reference strain (H37Rv) were investigated by Raman spectrometry with He-Ne (632,8 nm) laser excitation source. As a result of the research, the optimal experimental parameters have been obtained to get spectra of mycolic acids, which are part of the cell wall of mycobacteria.