Dr. Orly Liba Profile

Dr. Orly Liba

Research Scientist

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Publications (10)

Proceedings Article | 5 March 2021 Presentation

Quantitative hyperspectral microscopy for evaluating nanoparticle biodistribution in histological sections with single particle sensitivity

Elliott SoRelle, Orly Liba, Jos Campbell, Roopa Dalal, Cristina Zavaleta, Adam de la Zerda

Proceedings Volume 11624, 116240A (2021) https://doi.org/10.1117/12.2579530

KEYWORDS: Nanoparticles, Particles, Tissues, Microscopy, Plasmonics, Spleen, Liver, Kidney, Scattering, Nanorods

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Proceedings Article | 4 March 2019 Presentation

Gold nanoprisms enhanced optical coherence tomography angiography of live animals in the second near infrared window (Conference Presentation)

Peng Si, Edwin Yuan, Orly Liba, Yonatan Winetraub, Siavash Yousefi, Elliott SoRelle, Derek Yecies, Rebecca Dutta, Adam de la Zerda

Proceedings Volume 10867, 108671K (2019) https://doi.org/10.1117/12.2511528

KEYWORDS: Optical coherence tomography, Angiography, Near infrared, Gold, Tumors, In vivo imaging, Tissue optics, Scattering, Skin, Melanoma

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Proceedings Article | 14 February 2018 Paper

Speckle-modulation for speckle reduction in optical coherence tomography

Orly Liba, Matthew Lew, Elliott SoRelle, Rebecca Dutta, Debasish Sen, Darius Moshfeghi, Steven Chu, Adam de la Zerda

Proceedings Volume 10483, 104832D (2018) https://doi.org/10.1117/12.2289405

KEYWORDS: Dermatology, Optical coherence tomography, Speckle, Speckle pattern, Image resolution, Cornea, Diagnostics

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Proceedings Article | 14 February 2018 Paper

Optical coherence tomography of lymphatic vessel endothelial hyaluronan receptors in vivo

Peng Si, Debasish Sen, Rebecca Dutta, Siavash Yousefi, Roopa Dalal, Yonatan Winetraub, Orly Liba, Adam de la Zerda

Proceedings Volume 10483, 1048326 (2018) https://doi.org/10.1117/12.2282890

KEYWORDS: Optical coherence tomography, In vivo imaging, Molecular imaging, Inflammation, Receptors, Phase contrast, Lymphatic system, Signal detection

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Proceedings Article | 3 March 2017 Presentation + Paper

Spectral contrast-enhanced optical coherence tomography for improved detection of tumor microvasculature and functional imaging of lymphatic drainage

Elliott SoRelle, Orly Liba, Debasish Sen, Adam de la Zerda

Proceedings Volume 10063, 100630T (2017) https://doi.org/10.1117/12.2254103

KEYWORDS: Optical coherence tomography, Lymphatic system, Functional imaging, In vivo imaging, Tissues, Gold, Molecular imaging, Signal detection, Tissue optics, Coherence (optics), Tumors, Particles, Detection and tracking algorithms, Blood, Speckle, Blood vessels

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Proceedings Article | 21 February 2017 Paper

A model for quantifying contrast enhancement in optical coherence tomography (OCT)

Yonatan Winetraub, Elliott SoRelle, Orly Liba, Adam de la Zerda

Proceedings Volume 10074, 100740J (2017) https://doi.org/10.1117/12.2254498

KEYWORDS: Optical coherence tomography, Signal detection, Particles, Scattering, Signal attenuation, Ultrasonography, Speckle, Blood, In vivo imaging, Mie scattering

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Proceedings Article | 17 February 2017 Paper

Machine learning-assisted hyperspectral analysis of plasmonic contrast agent microbiodistribution with single-particle sensitivity and sub-cellular resolution

Elliott SoRelle, Orly Liba, Jos Campbell, Roopa Dalal, Cristina Zavaleta, Adam de la Zerda

Proceedings Volume 10080, 100800L (2017) https://doi.org/10.1117/12.2254117

KEYWORDS: Image classification, Nanoparticles, Particles, Hyperspectral imaging, Machine learning, Biological research, Plasmonics, Microscopy, Biomedical optics, Therapeutic agents, In vivo imaging, Tissues, Tumors, Gold, Liver

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Proceedings Article | 17 February 2017 Presentation + Paper

High sensitivity contrast enhanced optical coherence tomography for functional in vivo imaging

Orly Liba, Elliott SoRelle, Debasish Sen, Adam de la Zerda

Proceedings Volume 10053, 100531D (2017) https://doi.org/10.1117/12.2248258

KEYWORDS: Optical coherence tomography, In vivo imaging, Lymphatic system, Tissues, Molecular imaging, Gold, Image resolution, 3D image processing, Particles, Cancer, Blood, Blood vessels, Tumors, Ear

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SPIE Journal Paper | 6 June 2016 Open Access

High-resolution contrast-enhanced optical coherence tomography in mice retinae

Debasish Sen, Elliott SoRelle, Orly Liba, Roopa Dalal, Yannis Paulus, Tae-Wan Kim, Darius Moshfeghi, Adam de la Zerda

JBO, Vol. 21, Issue 06, 066002, (June 2016) https://doi.org/10.1117/12.10.1117/1.JBO.21.6.066002

KEYWORDS: Optical coherence tomography, Retina, Blood vessels, Scattering, Injuries, Imaging systems, Hyperspectral imaging, In vivo imaging, Eye, Tissues

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Proceedings Article | 12 March 2015 Paper

Size dependence of gold nanorod stability: the need for customized surface chemistry

Elliott SoRelle, Orly Liba, Zeshan Hussain, Milan Gambhir, Adam de la Zerda

Proceedings Volume 9338, 933814 (2015) https://doi.org/10.1117/12.2080705

KEYWORDS: Particles, Coating, Biomedical optics, Absorbance, Photoacoustic spectroscopy, Chemistry, Transmission electron microscopy, Gold, Nanoparticles, Nanorods

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Nanoparticles can be synthesized in a wide array of shapes and sizes to suit specific biomedical applications in therapy and imaging. Prerequisite to such applications are particle stability in biological environments, non-toxicity, and facile conjugation of the particle surface with targeting biological moieties (such as antibodies). Here we report significant flaws in the common methods used to functionalize the surface of gold nanorods (GNRs) of larger-than-usual sizes. We find that while GNRs of sizes smaller than 50 x 15 nm can be effectively stabilized by polyethylene glycol (PEG)-based methods, larger GNRs form major aggregates and crash under similar functionalization conditions. Large GNRs may provide enhanced imaging sensitivity in biological applications due to greater optical extinction cross sections, provided that the GNRs can be made biostable. In this study, GNRs of sizes up to 90 x 30 nm were synthesized using two different published methods. Particle morphology and size distributions were characterized using Transmission Electron Microscopy (TEM), and optical spectra were measured by Vis-NIR Spectrometry. The colloidal stability of different-sized GNRs was assayed at various stages of functionalization using zeta potential and Vis-NIR measurements. The results of these experiments indicate that large GNRs functionalized with PEG undergo irreversible aggregation after minimal washing. We find that coating large GNRs with polystyrene sulfonate (PSS) instead of PEG vastly improves GNR stability in water and serum. Moreover, we provide a novel platform for conjugating biomolecules of interest to PSS-coated large GNRs. We show that larger GNRs produce stronger photoacoustic signal than commonly used smaller GNRs, indicating an advantage of using large GNRs for biomedical imaging. Our observations underscore that the biomedical advantages of novel nanoparticle synthesis methods may not be realized without tailored surface functionalization methods. More generally, our results suggest that materially-identical nanoparticles (i.e. GNRs) exhibit varying stability as a function of particle size.

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