A new method is developed for recording and reconstructing a microscopic high-resolution 3-D image with no
distortion. Any imaging lens is not located between an object and a CCD in our optical system. An off-axis hologram
with a large numerical aperture is recorded at once, and a complex-amplitude in-line hologram is extracted from the
recorded off-axis hologram by applying the one-shot digital holography. A small complex-amplitude in-line hologram is
generated for the reconstruction of microscopic high-resolution images by dividing the large hologram into a number of
small holograms and by superimposing them. A focus-free image with high resolving power and no distortion is
reconstructed from the generated complex-amplitude in-line hologram. Resolution higher than 1μm is obtained in the
optical experiment, which can be improved up to the wave length. Microscopic high-resolution images with no distortion
can also be observed for objects immersed in the liquid or in the solid by using the lens-less holographic microscope.
A new method is developed for recording and reconstructing a microscopic high-resolution 3-D image with large depth.
Any imaging lens is not located between the object and the CCD in our optical system. A number of partial off-axis
holograms are sequentially recorded with one CCD, or are recorded at once with multi-channel CCD by the single- shot
digital holography. Partial complex-amplitude in-line holograms are extracted from recorded off-axis holograms by
applying the simple-shot digital holography, and are arranged in order to generate a wide complex-amplitude in-line
hologram. A high-resolution image with no distortion is numerically reconstructed from the wide complex-amplitude inline
A new method of the lens-less holographic microscope is proposed for recording a high-resolution 3-D image with large
visual depth. The light beam with a large viewing angle diffracted from the object surface is recorded by illuminating the
three-dimentional object with a large incidence angle. A number of partial holograms are sequentially recorded with one
CCD located at the fixed position by changing an incident angle of illumination light. These partial holograms are
arranged in order to generate a hologram with large viewing-zone angle for the reconstruction of a high-resolution
image. Optical experiments are carried out to demonstrate the present new method of the holographic microscope.
Results show that a high-resolution image is reconstructed from the wide hologram generated from a number of partial
holograms and the resolving power of reconstructed image is improved as the number of partial holograms becomes