Ms. Pakajiraporn Arunngam Profile

Pakajiraporn Arunngam

at Kwansei Gakuin University

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Proceedings Article | 20 February 2018 Paper

Study of the cell activity in three-dimensional cell culture by using Raman spectroscopy

Pakajiraporn Arunngam, Anggara Mahardika, Matsuyoshi Hiroko, Bibin Bintang Andriana, Yasuhiko Tabata, Hidetoshi Sato

Proceedings Volume 10497, 1049726 (2018) https://doi.org/10.1117/12.2288242

KEYWORDS: Raman spectroscopy, 3D image processing, 3D metrology, 3D modeling, Principal component analysis, Microscopes

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The purpose of this study is to develop a estimation technique of local cell activity in cultured 3D cell aggregate with gelatin hydrogel microspheres by using Raman spectroscopy. It is an invaluable technique allowing real-time, nondestructive, and invasive measurement. Cells in body generally exist in 3D structure, which physiological cell-cell interaction enhances cell survival and biological functions. Although a 3D cell aggregate is a good model of the cells in living tissues, it was difficult to estimate their physiological conditions because there is no effective technique to make observation of intact cells in the 3D structure. In this study, cell aggregates were formed by MC3T-E1 (pre-osteoblast) cells and gelatin hydrogel microspheres. In appropriate condition MC3T-E1 cells can differentiate into osteoblast. We assume that the activity of the cell would be different according to the location in the aggregate because the cells near the surface of the aggregate have more access to oxygen and nutrient. Raman imaging technique was applied to measure 3D image of the aggregate. The concentration of the hydroxyapatite (HA) is generated by osteoblast was estimated with a strong band at 950-970 cm^-1 which assigned to PO₄^3- in HA. It reflects an activity of the specific site in the cell aggregate. The cell density in this specific site was analyzed by multivariate analysis of the 3D Raman image. Hence, the ratio between intensity and cell density in the site represents the cell activity.

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