Prof. Paul M. W. French Profile

Prof. Paul M. W. French

Head of Photonics Group at Imperial College London

SPIE Involvement:

Conference Program Committee | Author

Publications (50)

PROCEEDINGS ARTICLE | April 24, 2017

Functional imaging of live Zebrafish using fluorescence lifetime optical projection tomography (Conference Presentation)

Proc. SPIE. 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV

KEYWORDS: X-ray optics, 3D image reconstruction, Luminescence, Biosensors, Tomography, Projection systems, Functional imaging, Fluorescence lifetime imaging, Optical tomography, Fluorescence resonance energy transfer

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PROCEEDINGS ARTICLE | April 27, 2016

Three-dimensional fluorescence imaging by stage-scanning oblique plane microscopy (Conference Presentation)

Proc. SPIE. 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII

KEYWORDS: Microscopes, 3D acquisition, Tumors, Cameras, Stereoscopy, Microscopy, Luminescence, Objectives, Fluorescence resonance energy transfer, 3D image processing

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PROCEEDINGS ARTICLE | November 19, 2014

Nanoparticle-assisted STED, theory, and experimental demonstration (presentation video)

Proc. SPIE. 9169, Nanoimaging and Nanospectroscopy II

KEYWORDS: Super resolution, Nanoimaging, Nanoparticles, Metals, Video, Particles, Luminescence, Stimulated emission depletion microscopy, Nanospectroscopy, Current controlled current source

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PROCEEDINGS ARTICLE | March 17, 2014

Autofluorescence lifetime metrology for label-free detection of cartilage matrix degradation

Proc. SPIE. 8940, Optical Biopsy XII

KEYWORDS: Microscopes, Metrology, Cartilage, Tissues, Microscopy, Fluorometers, Luminescence, Collagen, Scanning electron microscopy, Fluorescence lifetime imaging

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PROCEEDINGS ARTICLE | May 8, 2012

Towards gene therapy based on femtosecond optical transfection

Proc. SPIE. 8427, Biophotonics: Photonic Solutions for Better Health Care III

KEYWORDS: Femtosecond phenomena, Medicine, Tissues, Luminescence, Heart, Objectives, In vivo imaging, Laser optics, Plasma, Neurons

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PROCEEDINGS ARTICLE | June 14, 2011

Non-invasive imaging of skin cancer with fluorescence lifetime imaging using two photon tomography

Proc. SPIE. 8087, Clinical and Biomedical Spectroscopy and Imaging II

KEYWORDS: Hyperspectral imaging, Tissues, Image segmentation, Spectroscopy, Skin, Luminescence, Diagnostics, Skin cancer, Fluorescence lifetime imaging, In vivo imaging

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PROCEEDINGS ARTICLE | March 1, 2011

An automated wide-field time-gated optically sectioning fluorescence lifetime imaging multiwell plate reader for high-content analysis of protein-protein interactions

Proc. SPIE. 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII

KEYWORDS: Proteins, Rhodamine B, Data modeling, Image segmentation, Luminescence, Fluorescence lifetime imaging, Picosecond phenomena, Biological research, Fluorescence resonance energy transfer, Plasma

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PROCEEDINGS ARTICLE | March 1, 2011

A multispectral FLIM tomograph for in-vivo imaging of skin cancer

Proc. SPIE. 7903, Multiphoton Microscopy in the Biomedical Sciences XI

KEYWORDS: Hyperspectral imaging, Detection and tracking algorithms, Sensors, Image segmentation, Spectroscopy, Skin, Luminescence, Image sensors, Fluorescence lifetime imaging, In vivo imaging

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PROCEEDINGS ARTICLE | March 1, 2011

A STED-FLIM microscope applied to imaging the natural killer cell immune synapse

Proc. SPIE. 7903, Multiphoton Microscopy in the Biomedical Sciences XI

KEYWORDS: Confocal microscopy, Microscopes, Microscopy, Luminescence, Structured optical fibers, Spatial light modulators, Sapphire lasers, Fluorescence lifetime imaging, Spatial resolution, Stimulated emission depletion microscopy

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PROCEEDINGS ARTICLE | February 22, 2011

Fluorescence lifetime imaging endoscopy

Proc. SPIE. 7893, Endoscopic Microscopy VI

KEYWORDS: Confocal microscopy, Endoscopy, Tissues, Luminescence, Endoscopes, Biopsy, Fluorescence lifetime imaging, In vivo imaging, Fluorescence resonance energy transfer, Tissue optics

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PROCEEDINGS ARTICLE | February 18, 2011

Fluorescence lifetime imaging of skin cancer

Proc. SPIE. 7883, Photonic Therapeutics and Diagnostics VII

KEYWORDS: Roads, Tissues, Skin, Luminescence, Photons, Diagnostics, Skin cancer, Photonics, Fluorescence lifetime imaging, Two photon excitation microscopy

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PROCEEDINGS ARTICLE | February 17, 2011

Hyperspectral fluorescence lifetime fibre probe spectroscopy for use in the study and diagnosis of osteoarthritis and skin cancer

Proc. SPIE. 7895, Optical Biopsy IX

KEYWORDS: Diffuse reflectance spectroscopy, Cartilage, Tissues, Spectroscopy, Luminescence, Reflectivity, Collagen, Skin cancer, In vivo imaging, Tissue optics

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PROCEEDINGS ARTICLE | February 27, 2010

Investigating fast enzyme-DNA kinetics using multidimensional fluorescence imaging and microfluidics

Proc. SPIE. 7593, Microfluidics, BioMEMS, and Medical Microsystems VIII

KEYWORDS: Confocal microscopy, Computational fluid dynamics, Microscopes, Microfluidics, Polarization, Luminescence, Fluorescence anisotropy, Computer simulations, Fluorescence lifetime imaging, Anisotropy

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PROCEEDINGS ARTICLE | February 26, 2010

Tomographic imaging of flourescence resonance energy transfer in highly light scattering media

Proc. SPIE. 7573, Biomedical Applications of Light Scattering IV

KEYWORDS: Proteins, Scattering, Luminescence, Quantum efficiency, Calcium, Light scattering, Tomography, Resonance energy transfer, Fluorescence lifetime imaging, Fluorescence resonance energy transfer

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PROCEEDINGS ARTICLE | February 24, 2010

tomoFLIM: fluorescence lifetime projection tomography

Proc. SPIE. 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII

KEYWORDS: Luminescence, Calcium, 3D modeling, Tomography, Head, Reconstruction algorithms, Fluorescence lifetime imaging, Fluorescent proteins, Fluorescence resonance energy transfer, 3D image processing

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Optical Projection Tomography (OPT) is a wide-field technique for measuring the threedimensional distribution of absorbing/fluorescing species in non-scattering (optically cleared) samples up to ~1cm in size, and as such is the optical analogue of X-ray computed tomography. We have extended the intensity-based OPT technique to measure the three-dimensional fluorescence lifetime distribution (tomoFLIM) in transparent samples. Due to its inherent ratiometric nature, fluorescence lifetime measurements are robust against intensity-based artifacts as well as producing a quantitative measure of the fluorescence signal, making it particularly suited to Förster Resonance Energy Transfer (FRET) measurements. We implement tomoFLIM via OPT by acquiring a series of wide-field time-gated images at different relative time delays with respect to a train of excitation pulses for a range of projection angles. For each time delay, the three-dimensional time-gated intensity distribution is reconstructed using a filtered back projection algorithm and the fluorescence lifetime is subsequently determined for each reconstructed horizontal plane by iterative fitting of an appropriate decay model. We present a tomographic reconstruction of a fluorescence lifetime resolved FRET calcium contruct, TN-L15 cytosol suspension, in a silicone phantom. This genetically encoded sensor, TN-L15, comprises the calcium-binding domain of Troponin C, flanked by the fluorophores cyan fluorescent protein and citrine. In the presence of calcium ions TN-L15 changes conformation bringing the two fluorophores into close proximity, resulting in FRET. We also present autofluorescence and fluorescently labelled tomoFLIM reconstructions of chick embryos, including a genetically encoded fluorophore TagRFP-T. The fluorophore was electroporated in ovo into the neural tube of the embryos, which were subsequently dissected two days post-electroporation, fixed in ethanol and optically cleared for OPT/tomoFLIM acquisition. The reconstructed 3-D fluorescence lifetime image provides contrast between the genetically labelled TagRFP-T and the emitted autofluorescence.

PROCEEDINGS ARTICLE | July 13, 2007

Applications of rapid time-gated hyperspectral FLIM: live cell imaging of membrane order and 6-D microscopy

Proc. SPIE. 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III

KEYWORDS: Confocal microscopy, Microscopes, Tissues, Microscopy, Luminescence, Data acquisition, Spectral resolution, Charge-coupled devices, Live cell imaging, Fluorescence lifetime imaging

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PROCEEDINGS ARTICLE | July 12, 2007

Fluorescence lifetime imaging through turbid media reconstructed in the Fourier domain using time-gated imaging data

Proc. SPIE. 6629, Diffuse Optical Imaging of Tissue

KEYWORDS: Scattering, Cameras, Luminescence, Quantum efficiency, Fourier transforms, CCD cameras, Tomography, Epoxies, Fluorescence lifetime imaging, Picosecond phenomena

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PROCEEDINGS ARTICLE | February 19, 2007

Development of a hyperspectral fluorescence lifetime imaging microscope and its application to tissue imaging

Proc. SPIE. 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V

KEYWORDS: Confocal microscopy, Hyperspectral imaging, Microscopes, Spectrographs, Tissues, Cameras, Sensors, Luminescence, Fluorescence lifetime imaging, Tissue optics

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PROCEEDINGS ARTICLE | February 14, 2007

A novel hyperspectral lifetime probe for autofluorescence

Proc. SPIE. 6433, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VII

KEYWORDS: Optical fibers, Optical filters, Proteins, Roads, Tissues, Sensors, Skin, Ultraviolet radiation, Luminescence, Mode conditioning cables

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PROCEEDINGS ARTICLE | February 14, 2007

Fluorescence lifetime imaging using light-emitting diodes

Proc. SPIE. 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV

KEYWORDS: Confocal microscopy, Light emitting diodes, LED lighting, Modulation, Digital filtering, Microscopy, Luminescence, Light sources and illumination, Fluorescence lifetime imaging, Picosecond phenomena

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PROCEEDINGS ARTICLE | February 14, 2007

Microscopy using micropixellated light emitting diodes

Proc. SPIE. 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV

KEYWORDS: Confocal microscopy, Microscopes, Light sources, Light emitting diodes, Cameras, Microscopy, Luminescence, CCD cameras, Solid state electronics, Line scan image sensors

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PROCEEDINGS ARTICLE | October 8, 2005

Fluorescence lifetime imaging microscopy using a tunable continuum source and a Nipkow disk confocal microscope

Proc. SPIE. 5860, Confocal, Multiphoton, and Nonlinear Microscopic Imaging II

KEYWORDS: Confocal microscopy, Microscopes, Photonic crystal fibers, Multiphoton microscopy, Microscopy, Luminescence, Crystals, Physics, Fluorescence lifetime imaging, Confocal fluorescent microscopy

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PROCEEDINGS ARTICLE | October 8, 2005

Application of hyperspectral fluorescence lifetime imaging to tissue autofluorescence: arthritis

Proc. SPIE. 5862, Diagnostic Optical Spectroscopy in Biomedicine III

KEYWORDS: Hyperspectral imaging, Statistical analysis, Cartilage, Tissues, Luminescence, Diagnostics, Physics, Head, Software development, Fluorescence lifetime imaging

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SPIE Journal Paper | September 1, 2005

Toward the clinical application of time-domain fluorescence lifetime imaging

Munro, Ian , McGinty, James , Galletly, Neil , Requejo-Isidro, Jose , Lanigan, Peter , Elson, Daniel , Dunsby, Christopher , Neil, Mark , Lever, M. John , Stamp, Gordon , French, Paul

JBO Vol. 10 Issue 05

KEYWORDS: Fluorescence lifetime imaging, Luminescence, Tissues, Endoscopes, Tissue optics, Endoscopy, Signal to noise ratio, Charge-coupled devices, Picosecond phenomena, Image intensifiers

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PROCEEDINGS ARTICLE | September 1, 2005

Video rate fluorescence lifetime imaging and fluorescence lifetime endoscopy

Proc. SPIE. 5864, Novel Optical Instrumentation for Biomedical Applications II

KEYWORDS: Endoscopy, Biomedical optics, Tissues, Video, Luminescence, Endoscopes, Fluorescence lifetime imaging, Picosecond phenomena, Tissue optics, Laparoscopy

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PROCEEDINGS ARTICLE | September 23, 2003

Photorefractive holography for real-time coherence gated imaging

Proc. SPIE. 5067, Saratov Fall Meeting 2002: Laser Physics and Photonics, Spectroscopy, and Molecular Modeling III; Coherent Optics of Ordered and Random Media III

KEYWORDS: Holography, Coherence (optics), Scattering media, Cameras, Optical coherence tomography, Video, Physics, Charge-coupled devices, Gated imaging, Signal detection

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PROCEEDINGS ARTICLE | July 8, 2003

Imaging of tumor necroses using full-frame optical coherence imaging

Proc. SPIE. 4956, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII

KEYWORDS: Coherence imaging, Holograms, Holography, Biomedical optics, Tumors, Speckle, Optical coherence tomography, CCD cameras, Tissue optics, Four wave mixing

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PROCEEDINGS ARTICLE | July 8, 2003

Wide-field coherence gated imaging: photorefractive holography and wide-field coherent heterodyne imaging

Proc. SPIE. 4956, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII

KEYWORDS: Coherence imaging, Holography, Coherence (optics), Polarization, Scattering, Optical coherence tomography, Light scattering, CCD cameras, Charge-coupled devices, Signal detection

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PROCEEDINGS ARTICLE | June 14, 2002

Low-coherence photorefractive holography for high-speed 3D imaging including through scattering media

Proc. SPIE. 4619, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications VI

KEYWORDS: Microscopes, Mirrors, Holography, Coherence (optics), Modulation, Scattering, Stereoscopy, Light scattering, CCD cameras, Charge-coupled devices

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PROCEEDINGS ARTICLE | June 14, 2002

Optical coherence imaging of rat tumor spheroids

Proc. SPIE. 4619, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications VI

KEYWORDS: Coherence imaging, Beam splitters, Holograms, Holography, Biomedical optics, Tumors, Cameras, Optical coherence tomography, Video, Four wave mixing

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PROCEEDINGS ARTICLE | June 11, 2002

Autocorrelation imaging of 3D structures using a femtosecond laser: application to imaging of sandstone

Proc. SPIE. 4643, Ultrafast Phenomena in Semiconductors VI

KEYWORDS: Coherence imaging, Holograms, Holography, Reflection, Optical coherence tomography, Light scattering, Scanning electron microscopy, Translucency, Geometrical optics, 3D image processing

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PROCEEDINGS ARTICLE | May 31, 2002

High-speed 3-D imaging using photorefractive holography with novel low-coherence interferometers

Proc. SPIE. 4705, Saratov Fall Meeting 2001: Coherent Optics of Ordered and Random Media II

KEYWORDS: Microscopes, Prisms, Beam splitters, Holography, 3D image reconstruction, Coherence (optics), Scattering media, Stereoscopy, Luminescence, Light scattering

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PROCEEDINGS ARTICLE | November 2, 2001

Application of the stretched exponential function to fluorescence lifetime imaging of biological tissue

Proc. SPIE. 4431, Photon Migration, Optical Coherence Tomography, and Microscopy

KEYWORDS: Mathematical modeling, Proteins, Data modeling, Tissues, Luminescence, Collagen, Image quality, Fluorescence lifetime imaging, Tissue optics, Statistical modeling

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PROCEEDINGS ARTICLE | November 2, 2001

Five-dimensional fluorescence microscopy

Proc. SPIE. 4431, Photon Migration, Optical Coherence Tomography, and Microscopy

KEYWORDS: Microscopes, Biomedical optics, Optical spheres, Tissues, Imaging systems, Microscopy, Luminescence, Multispectral imaging, Fluorescence lifetime imaging, Tissue optics

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PROCEEDINGS ARTICLE | October 24, 2001

High-speed 3D imaging using photorefractive holography with novel low-coherence interferometers

Proc. SPIE. 4434, Hybrid and Novel Imaging and New Optical Instrumentation for Biomedical Applications

KEYWORDS: Holography, Light emitting diodes, Modulation, Imaging systems, Stereoscopy, Luminescence, Light scattering, Semiconductor lasers, CCD cameras, Tissue optics

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PROCEEDINGS ARTICLE | April 13, 2001

High-speed 3D imaging using photorefractive holography

Proc. SPIE. 4298, Three-Dimensional Image Capture and Applications IV

KEYWORDS: Holograms, Holography, Light emitting diodes, Imaging systems, Scattering, Stereoscopy, Light scattering, Image acquisition, Semiconductor lasers, 3D image processing

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PROCEEDINGS ARTICLE | November 22, 2000

Time-gated holographic imaging using photorefractive media

Proc. SPIE. 4160, Photon Migration, Diffuse Spectroscopy, and Optical Coherence Tomography: Imaging and Functional Assessment

KEYWORDS: Laser sources, Prisms, Holograms, Holography, Light emitting diodes, Coherence (optics), Scattering, Optical coherence tomography, Light scattering, Laser scattering

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PROCEEDINGS ARTICLE | April 6, 2000

Whole-field coherent imaging through turbid media using photorefractive holography

Proc. SPIE. 4001, Saratov Fall Meeting '99: Optical Technologies in Biophysics and Medicine

KEYWORDS: Holograms, Holography, Light emitting diodes, 3D image reconstruction, Tissues, Imaging systems, Speckle, Scattering, Video, Light scattering

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PROCEEDINGS ARTICLE | June 4, 1999

Fluorescence lifetime imaging for biomedicine using all-solid state ultrafast laser technology

Proc. SPIE. 3616, Commercial and Biomedical Applications of Ultrafast Lasers

KEYWORDS: Tissues, Imaging systems, Glasses, Luminescence, Laser applications, Amplifiers, Laser ablation, Fluorescence lifetime imaging, Picosecond phenomena, Laser systems engineering

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PROCEEDINGS ARTICLE | April 24, 1998

High-resolution real-time 3D imaging using time-gated photorefractive holography

Proc. SPIE. 3251, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications II

KEYWORDS: Holograms, Holography, 3D image reconstruction, Tissues, Imaging systems, Scattering, Scattering media, Video, Light scattering, 3D image processing

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PROCEEDINGS ARTICLE | April 16, 1998

Two-dimensional fluorescence lifetime imaging for in-vitro and in-vivo application

Proc. SPIE. 3250, Optical Biopsy II

KEYWORDS: Tissues, Imaging systems, Sensors, Luminescence, Bioalcohols, Collagen, Fluorescence lifetime imaging, Picosecond phenomena, Tissue optics, In vitro testing

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PROCEEDINGS ARTICLE | January 13, 1998

High-resolution whole field fluorescence lifetime imaging of fluorophore distribution and environment

Proc. SPIE. 3196, Optical and Imaging Techniques for Biomonitoring III

KEYWORDS: Proteins, Tissues, Imaging systems, Luminescence, Temporal resolution, Fluorescence lifetime imaging, Image intensifiers, Picosecond phenomena, Environmental sensing

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PROCEEDINGS ARTICLE | January 13, 1998

Time-gated holographic imaging using photorefractive media

Proc. SPIE. 3196, Optical and Imaging Techniques for Biomonitoring III

KEYWORDS: Ultrafast phenomena, Holography, Ferroelectric materials, Quantum wells, Real time imaging, Coherence (optics), Scattering, 3D image processing

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PROCEEDINGS ARTICLE | November 1, 1997

Novel ultrafast tunable solid state lasers for real-world applications including medical imaging

Proc. SPIE. 3176, Tunable Solid State Lasers

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PROCEEDINGS ARTICLE | May 22, 1997

Time-gated holographic imaging using photorefractive multiple quantum well devices

Proc. SPIE. 2981, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications

KEYWORDS: Holograms, Holography, Quantum wells, 3D image reconstruction, Imaging systems, Scattering, Scattering media, Light scattering, Image resolution, Absorption

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PROCEEDINGS ARTICLE | May 7, 1997

Two-dimensional fluorescence-lifetime imaging using a 5-kHz/110-ps gated image intensifier

Proc. SPIE. 2980, Advances in Fluorescence Sensing Technology III

KEYWORDS: Proteins, Tissues, Imaging systems, Luminescence, Temporal resolution, Fluorescence lifetime imaging, Image intensifiers, Picosecond phenomena, Tissue optics, Environmental sensing

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PROCEEDINGS ARTICLE | November 26, 1996

Depth-resolved holography using photorefractive media

Proc. SPIE. 2928, Biomedical Systems and Technologies

KEYWORDS: Holograms, Holography, 3D image reconstruction, Coherence (optics), Scattering, Spatial filters, Crystals, Light scattering, Laser scattering, Diffraction gratings

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PROCEEDINGS ARTICLE | January 15, 1996

Time-gated holographic imaging using photorefractive media

Proc. SPIE. 2628, Optical and Imaging Techniques for Biomonitoring

KEYWORDS: Holograms, Holography, Imaging systems, Scattering, Crystals, Light scattering, Laser scattering, Image resolution, Laser crystals, Spatial resolution

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PROCEEDINGS ARTICLE | May 30, 1995

Time-gated holographic imaging using photorefractive materials

Proc. SPIE. 2389, Optical Tomography, Photon Migration, and Spectroscopy of Tissue and Model Media: Theory, Human Studies, and Instrumentation

KEYWORDS: Holograms, Holography, Coherence (optics), Scattering, Crystals, Light scattering, Laser scattering, Image resolution, Laser crystals, 3D image processing

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PROCEEDINGS ARTICLE | April 10, 1995

All solid state femtosecond laser oscillators and amplifiers

Proc. SPIE. 2377, Generation, Amplification, and Measurement of Ultrashort Laser Pulses II

KEYWORDS: Optical amplifiers, Femtosecond phenomena, Oscillators, Mode locking, Argon ion lasers, Solid state lasers, Amplifiers, Semiconductor lasers, Diodes, Laser systems engineering

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