We recently reported construction of a new type of optical nano-construct composed of genome-depleted plant infecting
brome mosaic virus (BMV) doped with Indocyanine green (ICG), an FDA-approved chromophore. We refer to these
constructs as optical viral ghosts (OVGs) since only the capsid protein (CP) subunits of BMV remain to encapsulate
ICG. To utilize OVGs as effective nano-probes in fluorescence imaging applications, their fluorescence quantum yield
needs to be maximized. In this study, we investigate the effect of altering the CP to ICG mass ratio on the fluorescent
quantum yield of OVGs. Results of this study provide the basis for construction of OVGs with optimal amounts of CP
and ICG to yield maximal fluorescence quantum yield.