Ablative fractional skin laser is widely applied for various skin conditions, especially for cosmetic repairing and promoting the located drug delivery. Although the influence of laser treatment over the skin has been explored before in means of excision and biopsy with microscopy, these approaches are invasive, only morphological and capable of distorting the skin. In this paper the authors use fresh porcine skin samples irradiated by the lasers, followed by detected by using Optical Coherence Tomography (OCT). This advanced optical technique has the ability to present the high resolution structure image of treated sample. The results shows that laser beams can produce holes left on the surface after the irradiation. The depth of holes can be affected by changes of laser energy while the diameter of holes have no corresponding relation. Plus, OCT, as a valuable imaging technology, is capable of monitoring the clinical therapy procedure and assisting the calibration.
Regenerative medicine has the capability to revolutionise many aspects of medical care, but for it to make the step from
small scale autologous treatments to larger scale allogeneic approaches, robust and scalable label free cell sorting
technologies are needed as part of a cell therapy bioprocessing pipeline. In this proceedings we describe several
strategies for addressing the requirements for high throughput without labeling via: dimensional scaling, rare species
targeting and sorting from a stable state. These three approaches are demonstrated through a combination of optical and
ultrasonic forces. By combining mostly conservative and non-conservative forces from two different modalities it is
possible to reduce the influence of flow velocity on sorting efficiency, hence increasing robustness and scalability. One
such approach can be termed "optically enhanced acoustophoresis" which combines the ability of acoustics to handle
large volumes of analyte with the high specificity of optical sorting.
We report the combined use of optical sorting and acoustic levitation to give particle sorting. Differing sizes of microparticles are sorted optically both with and without the aid of acoustic levitation, and the results compared to show that the use of acoustic trapping can increase sorting efficiency. The use of a transparent ultrasonic transducer is also shown to streamline the integration of optics and acoustics. We also demonstrate the balance of optical radiation pressure and acoustic levitation to achieve vertical sorting.