We present three optical multi-channels spectrometers for the interrogation of label-free biosensors based on different kinds of transducers : resonant nanopillars (RNP), microring resonators (MRR), localized and propagative surface plasmon resonance (LSPR and SPR). Light is collected from the multi-channel biosensors (up to 12-channels) with optical fibers and is remapped to a packed straight line forming the input slit of the spectrometers. The combination of high resolution CMOS sensors and embedded signal processing makes it possible to extract the resonant wavelengths of the transducers with a precision in the range of 1-20 pm depending on the type of transducer. The performance of the three transducer / spectrometer systems has been evaluated in the framework of EU and regional projects for the monitoring of chemical pollutants found in oceanic waters (FP7 - EnviGuard), crop health monitoring (Interreg France-Wallonie-Vlaanderen - SmartBioControl/BioSens) and bioreactor monitoring (EutoTransBio - APTACHIP).
In recent works it has been demonstrated the suitability of using resonant nanopillars (R-NPs) as biochemical. In this work it has been shown the capability of the R-NPs to behave as label-free multiplexed biological sensors. Each R-NP is formed by silicon oxide (SiO2) and silicon nitride (Si3N4) Bragg reflectors and a central cavity of SiO<sub>2</sub>, and they are grouped into eight arrays called BICELLs, which are distributed on a single chip of quartz substrate for multiplexing measurements. For the biological sensing assessment it was developed an immunoassay on the eight single BICELLs. The biofunctionalization process was performed by a silanization protocol based on 3-aminopropyltrymethoxysilane (APTMS) and glutaradheyde (GA) as a linker between APTMS and the IgG which acted as biorreceptor for the anti-IgG recognition. In this work, there were compared two forms of immobilization: on one hand by incubating the R-NPs under static drop of 50 μg/mL and on the second hand by introducing the sensing chip in a flow cell with a continuous flow of the same concentration of IgG. The eight arrays of R-NPs or BICELLs were independently optically interrogated by a bundle of fiber connected to a spectrometer. The multiplexing analysis showed reproducibility among the BICELLs, suggesting the potentially of using R-NPs for multiplexed biosensors. Performance in the immobilization process apparently does not have a signification effect. However the election of one method or another should be a commitment between time and resources.