Dynamic Light Scattering is used for measuring particle size distribution of nano-particle under Brownian motion. Signal is detected through a photomultiplier and processed by correlation analysis, and results are inverted at last. Method by using CCD camera can record the procedure of motion. However, there are several weaknesses such as low refresh speed and noise from CCD camera, and this method depends on particle size and detecting angle. A simulation of nano-particle under Brownian motion is proposed to record dynamic images, studies contrast of dynamic images which can represent speed of diffusion, and its characteristic under different conditions. The results show that through contrast of dynamic images diffusion coefficient can be obtained, which is independent on density of scattering volume.