The use of terahertz radiation in various fields such as biology and medicine is increasing every year. Meanwhile, people are increasingly concerned about the mechanism on the interaction between terahertz radiation and biological system. In this study, we evaluated the effect of the cellular response of bEnd.3 which exposing to the terahertz radiation in the range of 0.1-3.5 THz. We collected the spectral data of cells during the irradiation with a temperature of 20.6°C and a relative humidity of 5%. Meanwhile, the apoptosis of cell was assessed by Annexin V-FITC/PI kit. As a result, the apoptosis of cells were inhibited from 0 and 6 hours after terahertz irradiation and promoted at 12 hours after irradiation. Moreover, we also calculated the complex dielectric constant of bEnd.3 cells at different exposures. The results demonstrate that the dielectric loss of cells showed a slight decrease with the increase of exposure in the range of 0.2-1.4 THz.
As a new two-dimensional material, MoS2 has been extensively studied in terahertz modulation due to its excellent photoelectric properties. Herein, we studied a novel method for label-free detection of the carcinoembryonic antibody based on MoS2 nanosheets using terahertz spectroscopy. Protein molecules were fabricated on the COC substrates with MoS2 nanosheets. A self-made simple sample cell was used to get signal of protein molecules in a liquid phase. Computational analysis was performed using a classic sandwich structure model. The refractive index and absorption coefficient were calculated. As a result, the THz signal increased with the protein concentration increasing in 0.2-1.1THz. The cole-cole plot was also investigated. The effectiveness of MoS2 nanosheets for adsorbing protein molecules has been demonstrated, which can be used to detect carcinoembryonic antigen sensitively in the future.