We report the results of experimental studies on cardiac implants using a Raman spectroscopy method (RS). Raman spectra characteristics of leaves and walls of cardiac implants were obtained; the implants were manufactured by protocols of detergent-enzymatic technique (DET) and biological, detergent-free (BIO) decellularization, using detergents (group DET) or a detergent-free, nonproteolytic, actin-disassembling regimen (BIO). There were input optical coefficients that allowed us to carry out evaluation of the protocols of DET and BIO decellularization on the basis of the concentrations of glycosaminoglycans, proteins, amides, and DNA. It was shown that during DET and BIO decellularization, composition aberrations of proteins and lipids do not occur and the integrity of the collagenous structures is preserved. It was found that during the DET decellularization, preservation of glycosaminoglycans is better than during BIO decellularization.
In this work are presented the results of experimental studies of biological tissue implants using the method of Raman spectroscopy (RS). Raman spectra were obtained particularly biological tissue implants made by two different protocols. Were introduced optic coefficients, to monitor the content of the main components in bio tissue implants in the manufacturing process and to assess the quality of their processing on the selected protocol.
In this work the results of cell-tissue grafts research with a complex of optical methods – confocal fluorescent microscopy and Raman spectroscopy are presented. It was established that coefficient M scatter is related to irregularity of demineralization process. It was microscopically shown that the quantity of integrated cells into these types of transplants amounts to 20% of its surface.
The results of experimental studies of donor bone samples (rat, rabbit and human) with varying degrees of
mineralisation by Raman spectroscopy were presented. Raman spectra were obtained for the Raman bands 950-962
(РО4)3-, 1065-1070 (СО3)2- and 1665 cm-1 (Amide I).
In demineralized bone a sharp decline (to 98 %) in the range of 950-962 cm-1 (РО4)3- and 1065 - 1070 cm-1 was
observed. This decrease was accompanied by the emergence of the 1079-1090 cm-1 band corresponding to the hydrated