Dr. Peter M. Goodwin Profile

Dr. Peter M. Goodwin

at Los Alamos National Lab

SPIE Involvement:

Author

Publications (30)

Proceedings Article | 15 March 2023 Presentation + Paper

Presentation + Paper

Time-resolved super-resolution microscopy to image photoluminescence lifetimes and spatially resolve dual emitter semiconductor nanostructures

Megan Dunlap, Liam Koch, Duncan Ryan, Peter Goodwin, James Werner, Paul Bourdin, Jennifer Hollingsworth, Chris Sheehan, Martin Gelfand, Alan Van Orden

Proceedings Volume 12386, 123860C (2023) https://doi.org/10.1117/12.2650337

KEYWORDS: Photons, Quantum emitters, Pulsed laser operation, Point spread functions, Super resolution, Semiconductors, Photoluminescence, Super resolution microscopy, Nanostructures, Time correlated single photon counting

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Proceedings Article | 15 March 2023 Presentation

Presentation

Super-resolution localization microscopy with orientation and spectral discrimination (Conference Presentation)

Duncan Ryan, Peter Goodwin, James Werner, Somak Majumder, Jennifer Hollingsworth, Megan Dunlap, Alan Van Orden, Martin Gelfand

Proceedings Volume PC12386, PC123860C (2023) https://doi.org/10.1117/12.2647902

KEYWORDS: Microscopy, Atmospheric particles, Super resolution, Quantum dots, Semiconductors, Particles, Optical microscopy, Nanolithography, Nanocrystals, Microscopes

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Proceedings Article | 9 March 2023 Presentation

Presentation

Biological quantum imaging with non-degenerate photon pairs

Duncan Ryan, James Werner, Kristina Meier, David Thompson, Peter Goodwin, Rebecca Holmes, Raymond Newell, Demosthenes Morales, David Hanson, Scott Twary, Kati Seitz

Proceedings Volume PC12447, PC1244738 (2023) https://doi.org/10.1117/12.2647903

KEYWORDS: Near infrared, Imaging devices, Photosynthesis, Photodetectors, Microscopes, Imaging technologies

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SPIE Journal Paper | 22 October 2022

Power spectral density analysis of a scaled model simulation of an optical tweezer

Vatsal Joshi, Peter Goodwin, Alan Bowling

OE, Vol. 61, Issue 10, 104103, (October 2022) https://doi.org/10.1117/12.10.1117/1.OE.61.10.104103

KEYWORDS: Optical tweezers, Motion models, Particles, Systems modeling, Differential equations, Optical engineering, Stochastic processes, Computing systems, Computer simulations, Motion analysis

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Proceedings Article | 13 February 2020 Presentation + Paper

Presentation + Paper

Single molecule localization with four avalanche photodiode detectors

Megan Dunlap, Duncan Ryan, Peter Goodwin, James Werner, Somak Majumder, Jennifer Hollingsworth, Martin Gelfand, Alan Van Orden

Proceedings Volume 11246, 112460O (2020) https://doi.org/10.1117/12.2546573

KEYWORDS: Sensors, Point spread functions, Monte Carlo methods, Optical fibers, Avalanche photodetectors, Avalanche photodiodes, Luminescence, Molecules, Time correlated photon counting, Super resolution microscopy, Fluorescence lifetime imaging, Single photon detectors, Single molecule spectroscopy, Quantum dots

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Proceedings Article | 13 February 2020 Presentation + Paper

Presentation + Paper

Dual-color super-resolution imaging of quantum dot clusters

Duncan Ryan, Megan Dunlap, Somak Majumder, Chris Sheehan, James Werner, Jennifer Hollingsworth, Martin Gelfand, Alan Van Orden, Peter Goodwin

Proceedings Volume 11246, 112460B (2020) https://doi.org/10.1117/12.2542352

KEYWORDS: Super resolution, Atmospheric particles, Quantum dots, Energy transfer, Luminescence, Beam splitters, Sensors, Fluorescence resonance energy transfer, Particles, Scanning electron microscopy

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SPIE Journal Paper | 25 October 2016 Open Access

Open Access

Light-sheet microscopy by confocal line scanning of dual-Bessel beams

Pengfei Zhang, Mary Phipps, Peter Goodwin, James Werner

JBO, Vol. 21, Issue 10, 100502, (October 2016) https://doi.org/10.1117/12.10.1117/1.JBO.21.10.100502

KEYWORDS: Bessel beams, Digital micromirror devices, Confocal microscopy, Luminescence, Microscopy, Imaging systems, Microscopes, Spatial filters, Mirrors, Photons

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SPIE Journal Paper | 29 September 2016 Open Access

Open Access

Super-resolution optical microscopy study of telomere structure

Mary Lisa Phipps, Peter Goodwin, Jennifer Martinez, Edwin Goodwin

JBO, Vol. 21, Issue 09, 094003, (September 2016) https://doi.org/10.1117/12.10.1117/1.JBO.21.9.094003

KEYWORDS: Optical microscopy, Super resolution, Proteins, Microscopy, Optical fibers, Luminescence, Electron microscopy, Molecules, Visualization, Glasses

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Proceedings Article | 1 March 2016 Presentation + Paper

Presentation + Paper

Correlating structure and fluorescence dynamics of quantum dot clusters using super-resolution imaging

Duncan Ryan, Peter Goodwin, Chris Sheehan, Kevin Whitcomb, Martin Gelfand, Alan Van Orden

Proceedings Volume 9714, 97140T (2016) https://doi.org/10.1117/12.2211150

KEYWORDS: Fluorescence resonance energy transfer, Luminescence, Super resolution, Energy transfer, Microscopy, Particles, Electron microscopy, Scanning electron microscopy, Quantum dots, Solar energy, Atmospheric particles, Denoising, Microscopes, Point spread functions

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Proceedings Article | 12 March 2015 Paper

Paper

Three dimensional time-gated tracking of non-blinking quantum dots in live cells

Matthew DeVore, Dominik Stich, Aaron Keller, Yagna Ghosh, Peter Goodwin, Mary Phipps, Michael Stewart, Cédric Cleyrat, Bridget Wilson, Diane Lidke, Jennifer Hollingsworth, James Werner

Proceedings Volume 9338, 933812 (2015) https://doi.org/10.1117/12.2082943

KEYWORDS: Luminescence, Microscopes, Quantum dots, Particles, Signal to noise ratio, Sensors, Proteins, Molecules, Receptors, Fluorescent proteins

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Proceedings Article | 4 March 2014 Paper

Paper

Fast, 3D imaging via confocal line scanning of a Bessel beam using a single galvo mirror

Pengfei Zhang, Peter Goodwin, James Werner

Proceedings Volume 8947, 89471K (2014) https://doi.org/10.1117/12.2036848

KEYWORDS: Luminescence, Bessel beams, Image filtering, Stereoscopy, Confocal microscopy, Cameras, 3D image processing, Mirrors, Microscopes, Optical filters

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Proceedings Article | 13 February 2012 Paper

Paper

"Sizing" the oligomers of Azami Green fluorescent protein with FCS and antibunching

Jamshid Temirov, James Werner, Peter Goodwin, Andrew Bradbury

Proceedings Volume 8228, 82280J (2012) https://doi.org/10.1117/12.906843

KEYWORDS: Fluorescence correlation spectroscopy, Molecules, Proteins, Fluorescent proteins, Green fluorescent protein, Luminescence, Diffusion, Fluorescence spectroscopy, Sensors, Molecular spectroscopy

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Proceedings Article | 11 February 2011 Paper

Paper

A photophysical study of two fluorogen-activating proteins bound to their cognate fluorogens

Tiziano Gaiotto, Hau Nguyen, Jaemyeong Jung, Gnana Gnanakaran, Jurgen Schmidt, Geoffrey Waldo, Andrew Bradbury, Peter Goodwin

Proceedings Volume 7905, 79050O (2011) https://doi.org/10.1117/12.875418

KEYWORDS: Proteins, Fluorescence correlation spectroscopy, Luminescence, Diffusion, Imaging spectroscopy, Molecules, Molecular spectroscopy, Avalanche photodetectors, Single molecule spectroscopy, Imaging systems

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Proceedings Article | 25 February 2009 Paper

Paper

Going beyond 2D: following membrane diffusion and topography in the IgE-Fc[epsilon]RI system using 3-dimensional tracking microscopy

Nathan Wells, Guillaume Lessard, Mary Phipps, Peter Goodwin, Diane Lidke, Bridget Wilson, James Werner

Proceedings Volume 7185, 71850Z (2009) https://doi.org/10.1117/12.809412

KEYWORDS: Diffusion, Receptors, Microscopes, Plasma, Microscopy, Quantum dots, Particles, Confocal microscopy, Molecules, Signal processing

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SPIE Journal Paper | 1 May 2008 Open Access

Open Access

Probing dimerization and intraprotein fluorescence resonance energy transfer in a far-red fluorescent protein from the sea anemone Heteractis crispa

Guillaume Lessard, Satoshi Habuchi, James Werner, Peter Goodwin, Frans De Schryver, Johan Hofkens, Mircea Cotlet

JBO, Vol. 13, Issue 03, 031212, (May 2008) https://doi.org/10.1117/12.10.1117/1.2937477

KEYWORDS: Chromophores, Luminescence, Green fluorescent protein, Fluorescence resonance energy transfer, Proteins, Fluorescence correlation spectroscopy, Fluorescent proteins, Fluorescence spectroscopy, Molecules, Diffusion

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Proceedings Article | 27 February 2006 Paper

Paper

Three-dimensional tracking of fluorescent particles

Guillaume Lessard, Peter Goodwin, James Werner

Proceedings Volume 6092, 609205 (2006) https://doi.org/10.1117/12.650191

KEYWORDS: Particles, Sensors, Monte Carlo methods, Molecules, Luminescence, Diffusion, Fluorescence correlation spectroscopy, Photons, 3D acquisition, Target detection

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Proceedings Article | 27 February 2006 Paper

Paper

Time-resolved detection of the one- and two-photon excited fluorescence of single molecules of a folding enhanced green fluorescent protein

Mircea Cotlet, Peter Goodwin, Geoffrey Waldo, James Werner

Proceedings Volume 6092, 609204 (2006) https://doi.org/10.1117/12.646697

KEYWORDS: Luminescence, Molecules, Green fluorescent protein, Signal detection, Chromophores, Fluorescence spectroscopy, Proteins, Molecular lasers, Absorption, Rhodamine

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Proceedings Article | 22 February 2006 Paper

Paper

Single molecule spectroscopic characterization of a far-red fluorescent protein (HcRed) from the Anthozoa coral Heteractis crispa

Mircea Cotlet, Satoshi Habuchi, Jennifer Whitier, James Werner, Frans De Schryver, Johan Hofkens, Peter Goodwin

Proceedings Volume 6098, 609804 (2006) https://doi.org/10.1117/12.646811

KEYWORDS: Luminescence, Chromophores, Proteins, Molecules, Molecular spectroscopy, Fluorescence spectroscopy, Green fluorescent protein, Absorption, Spectroscopy, Fluorescent proteins

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We report on the photophysical properties of a far-red intrinsic fluorescent protein by means of single molecule and ensemble spectroscopic methods. The green fluorescent protein (GFP) from Aequorea victoria is a popular fluorescent marker with genetically encoded fluorescence and which can be fused to any biological structure without affecting its function. GFP and its variants provide emission colors from blue to yellowish green. Red intrinsic fluorescent proteins from Anthozoa species represent a recent addition to the emission color palette provided by GFPs. Red intrinsic fluorescent markers are on high demand in protein-protein interaction studies based on fluorescence-resonance energy transfer or in multicolor tracking studies or in cellular investigations where autofluorescence possesses a problem. Here we address the photophysical properties of a far-red fluorescent protein (HcRed), a mutant engineered from a chromoprotein cloned from the sea anemone Heteractis crispa, by using a combination of ensemble and single molecule spectroscopic methods. We show evidence for the presence of HcRed protein as an oligomer and for incomplete maturation of its chromophore. Incomplete maturation results in the presence of an immature (yellow) species absorbing/fluorescing at 490/530-nm. This yellow chromophore is involved in a fast resonance-energy transfer with the mature (purple) chromophore. The mature chromophore of HcRed is found to adopt two conformations, a Transoriented form absorbing and 565-nm and non-fluorescent in solution and a Cis-oriented form absorbing at 590-nm and emitting at 645-nm. These two forms co-exist in solution in thermal equilibrium. Excitation-power dependence fluorescence correlation spectroscopy of HcRed shows evidence for singlet-triplet transitions in the microseconds time scale and for cis-trans isomerization occurring in a time scale of tens of microseconds. Single molecule fluorescence data recorded from immobilized HcRed proteins, all point to the presence of two classes of molecules: proteins with Cis and Trans-oriented chromophores. Immobilization of HcRed in water-filled pores of polyvinyl alcohol leads to a polymer matrix - protein barrel interaction which results in a 'freezing' of the chromophore in a stable conformation for which non-radiative deactivation pathways are either suppressed or reduced. As a result, proteins with both Cis- and Trans-oriented chromophores can be detected at the single molecule level. Polymer chain motion is suggested as a mediator for an eventual cis-trans isomerization of the chromophore in the case of single immobilized proteins.

Proceedings Article | 19 June 2003 Paper

Paper

Quantitative analysis of specific nucleic acid sequences by two-color single-molecule fluorescence detection

Peter Goodwin, Rhiannon Nolan, Hong Cai

Proceedings Volume 4962, (2003) https://doi.org/10.1117/12.488210

KEYWORDS: Luminescence, Fluorescence spectroscopy, Target detection, Photodetectors, Roentgenium, Molecules, Statistical analysis, Avalanche photodetectors, Control systems, Solids

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Proceedings Article | 18 June 2001 Paper

Paper

Observing single-molecule chemical reactions on metal nanoparticles

Steven Emory, W. Patrick Ambrose, Peter Goodwin, Richard Keller

Proceedings Volume 4258, (2001) https://doi.org/10.1117/12.430762

KEYWORDS: Surface enhanced Raman spectroscopy, Molecules, Nanoparticles, Silver, Carbon, Raman spectroscopy, Metals, Photochemistry, Glasses, Electromagnetism

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Proceedings Article | 3 May 1999 Paper

Paper

Current status of DNA sequencing by single molecule detection

James Werner, Hong Cai, Peter Goodwin, Richard Keller

Proceedings Volume 3602, (1999) https://doi.org/10.1117/12.347535

KEYWORDS: Molecules, Luminescence, Laser beam diagnostics, Molecular lasers, Laser development, Rhodamine, Laser induced fluorescence, Fluorescence spectroscopy, Error analysis, Optical tweezers

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Proceedings Article | 15 May 1998 Paper

Paper

Probing the heterogeneous fluorescence-lifetime behavior of single rhodamine-6G molecules on fused silica

W. Patrick Ambrose, Peter Goodwin, Joerg Enderlein, David Semin, Richard Keller

Proceedings Volume 3270, (1998) https://doi.org/10.1117/12.308379

KEYWORDS: Molecules, Luminescence, Silica, Near field scanning optical microscopy, Rhodamine, Molecular lasers, Microscopy, Single photon, Near field, Optical properties

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Proceedings Article | 7 May 1997 Paper

Paper

Modeling single-molecule detection statistics

Joerg Enderlein, David Robbins, W. Patrick Ambrose, Peter Goodwin, Richard Keller

Proceedings Volume 2980, (1997) https://doi.org/10.1117/12.273570

KEYWORDS: Molecules, Luminescence, Photons, Molecular lasers, Capillaries, Quantum efficiency, Statistical modeling, Absorption, Diffusion, Data modeling

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Proceedings Article | 15 April 1997 Paper

Paper

Near-field optical microscopy nanoarray

David Semin, W. Patrick Ambrose, Peter Goodwin, Joel Wendt, Richard Keller

Proceedings Volume 3009, (1997) https://doi.org/10.1117/12.271219

KEYWORDS: Near field scanning optical microscopy, Molecules, Near field, Luminescence, Microscopes, Scanning electron microscopy, Atomic force microscopy, Aluminum, Silica, Etching

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Proceedings Article | 3 April 1995 Paper

Paper

Flow-based continuous DNA sequencing via single molecule detection of enzymatically cleaved fluorescent nucleotides

Jay Schecker, Peter Goodwin, Rhett Affleck, Ming Wu, John Martin, James Jett, Richard Keller, John Harding

Proceedings Volume 2386, (1995) https://doi.org/10.1117/12.206012

KEYWORDS: Molecules, Capillaries, Copper, Microscopes, Chlorine, Photon counting, Photodetectors, Quartz, Statistical analysis, Solids

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Proceedings Article | 21 July 1994 Paper

Paper

Fluorescence detection of single molecules using pulsed near-field optical excitation and time-correlated photon counting

W. Patrick Ambrose, Peter Goodwin, John Martin, Richard Keller

Proceedings Volume 2125, (1994) https://doi.org/10.1117/12.180866

KEYWORDS: Molecules, Luminescence, Metals, Silica, Near field scanning optical microscopy, Photons, Near field, Signal to noise ratio, Near field optics, Time correlated photon counting

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Proceedings Article | 24 June 1993 Paper

Paper

Mechanisms of excimer laser ablation of strongly absorbing systems

Charles Otis, Bodil Braren, Michael Thompson, D. Brunco, Peter Goodwin

Proceedings Volume 1856, (1993) https://doi.org/10.1117/12.147610

KEYWORDS: Polymers, Laser ablation, Polymer thin films, Absorption, Dye lasers, Excimer lasers, Temperature metrology, Image quality, Systems modeling, Ultraviolet radiation

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Proceedings Article | 24 June 1993 Paper

Paper

Ultrasensitive detection of single molecules in flowing sample streams by laser-induced fluorescence

Peter Goodwin, Charles Wilkerson, W. Patrick Ambrose, Richard Keller

Proceedings Volume 1895, (1993) https://doi.org/10.1117/12.146731

KEYWORDS: Molecules, Luminescence, Molecular lasers, Fluorescence spectroscopy, Pulsed laser operation, Diagnostics, Solids, Monte Carlo methods, Diffusion, Laser induced fluorescence

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Proceedings Article | 24 June 1993 Paper

Paper

Sizing of DNA fragments by flow cytometry

Mitchell Johnson, Peter Goodwin, W. Patrick Ambrose, John Martin, Babetta Marrone, James Jett, Richard Keller

Proceedings Volume 1895, (1993) https://doi.org/10.1117/12.146730

KEYWORDS: Luminescence, Diagnostics, Flow cytometry, Raman scattering, Fluorescence spectroscopy, Statistical analysis, Raman spectroscopy, Mode locking, Laser induced fluorescence, Molecules

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Proceedings Article | 24 June 1993 Paper

Paper

DNA sequencing by single molecule detection of labeled nucleotides sequentially cleaved from a single strand of DNA

Peter Goodwin, Jay Schecker, Charles Wilkerson, Mark Hammond, W. Patrick Ambrose, James Jett, John Martin, Babetta Marrone, Richard Keller, Alberto Haces, Po-Jen Shih, John Harding

Proceedings Volume 1891, (1993) https://doi.org/10.1117/12.146712

KEYWORDS: Molecules, Polymers, Laser development, Luminescence, Biological research, Molecular biology, Analog electronics, Rhodamine, Optical spheres, Sensors

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Showing 5 of 30 publications

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