We present results from our Ho:YLF regenerative amplifier (RA) producing up to 6.9 mJ at a repetition rate of 1 kHz and up to 12.9 mJ at a repetition rate of 10 Hz. At 1 kHz, we observe strongly bifurcating pulses, starting with certain round trip number, but the measurements identify a highly stable operation point that lies “hidden” beyond the instability region. This operation point allows the extraction of highly stable and high energetic output pulses. Suppression of bifurcation in our system is presented for repetition rates below 750 Hz and Ho:YLF crystal holder temperatures of 2.5 °C. We furthermore present a stability optimization routine for our Ho:YLF RA that was operated close to gain depletion at a repetition rate of 100 Hz. By varying the Ho:YLF crystal holder temperature the gain depletion level could be fine adjusted, resulting in a highly stable RA system with measured pulse fluctuations of only 0.35 %.