Surface plasmon resonance (SPR) technique is an optical method that allows the real time detection of small
changes in the physical properties (in particular the refractive index) of a dielectric medium near a metallic surface. This
technique is today applied to the realization of dynamic optical biochips where multiple interactions can be monitored in
parallel and in real time. One of the main advantage compared to other techniques as fluorescence detection is that it
does not require the presence of labels, which could influence the kinetics or the equilibrium of the biomolecular
interactions. However, as the SPR signal amplitude depends on the refractive index shift of the dielectric medium in the
contact with the metallic layer, one way to increase the SPR signal shift is to incorporate a substance possessing a strong
dispersive refractive index. We present the influence of organic chromophores incorporated in the DNA target molecules
on the spectral SPR response of a SPR sensor. Theoretical and experimental results are presented, showing that the DNA
target molecules labeled with chromophores presenting strong spectral refractive index variation in the spectral range of
the SPR spectrum induce significant spectral SPR response changes. The use of specific chromophores provides a
potential way of SPR response enhancement and initial results suggest that this phenomenon can also be used in realtime
SPR imaging detection.
Sensors based on surface plasmon resonance have the potential to provide information on the binding of biological
molecules on adequate substrates over typically thousand channels in parallel, without the need for any marker and in
real time compared to the scale of biochemical reactions. The need to optimize selectivity and sensitivity has triggered
continued research efforts. We review those related with optics and image processing, at the same time identifying some
aspects that deserve further investigation before the potential of the technique is fully utilized.
We have realized a surface plasmon resonance imaging system allowing accurate characterization of biochips. In this paper, the Rouard approach is extended to absorbing layers to model the reflectivity information contained in the multidimensional data. The multidimension potential is also expressed to demonstrate the power of the SPR imaging system. To conclude, towards the development of a biosensor based on SPR, a theoretical study is also performed on the sensitivity to changes in reflectivity of such multidimension optical biosensor. The sensitivity of the sytem shows the power of this biophotonic technology.
Surface plasmon resonance imaging is an optical method that allows the real time detection of small changes in the physical properties of a dielectric medium near a metallic surface. Using proper surface functionalization and structuration, this technique can be applied to the realization of optical biochips where multiple unlabeled interactions can be monitored. More precisely, thanks to the use of an adequate optical set-up built around a gold surface realized by self assembled monolayers or electrocopolymerization, we studied DNA:DNA interactions with potential application to genetic diagnostic and DNA:protein interactions to demonstrate the ability of the system to determine simultaneously different affinity constants.
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