Since the spectral imaging technology emerged, it has gained a lot of application achievements in the military field,
precision agriculture and biomedical science. When the fluorescence spectrum imaging first applied to the detection of
the feature resource of Chinese herbal medicine, the characteristics of holistic and ambiguity made it a new approach to
the traditional Chinese medicine testing. In this paper, we applied this method to study the Chinese medicine north and
south isatis root granules by comparing their fluorescence spectra. Using cluster analysis, the results showed that the
north and south Banlangen can not be divided by ascription. And these indicate that there is a large difference in the
quality of Banlangen granules on the market, and fluorescence spectrum imaging method can be used in monitoring the
quality of radix isatidis granules.