Mr. Rainer Erdmann Profile

Rainer Erdmann

Managing Director at PicoQuant GmbH

SPIE Involvement:

Conference Chair | Editor | Author

Publications (81)

Proceedings Article | 13 March 2024 Presentation

Small SPAD-arrays for confocal fluoresence lifetime imaging

Felix Koberling, Max Tillmann, Tino Roehlicke, Michael Wahl, Rainer Erdmann, Ivan Michel Antolovic

Proceedings Volume PC12847, PC128470Y (2024) https://doi.org/10.1117/12.3001460

KEYWORDS: Confocal microscopy, Time correlated single photon counting, Fluorescence lifetime imaging, Fluorescence correlation spectroscopy, Super resolution, Single photon detectors, Imaging technologies, Data processing

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Proceedings Article | 13 March 2024 Presentation

Luminosa: making confocal fluorescence microscopy a tool for every biophysicist

Felix Koberling, Fabio Barachati, Marcelle Koenig, Maria Loidolt-Krueger, Ellen Schmeyer, Matthias Patting, Marcus Sackrow, Uwe Ortmann, Evangelos Sisamakis, Rainer Erdmann

Proceedings Volume PC12847, PC1284710 (2024) https://doi.org/10.1117/12.3006371

KEYWORDS: Confocal microscopy, Fluorescence microscopy, Fluorescence lifetime imaging, Statistical analysis, Image analysis, Time resolved spectroscopy, Statistical modeling, Reproducibility, Process modeling, Photon counting

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Proceedings Article | 13 March 2024 Presentation

Bringing time-resolved fluorescence microscopy to a broader research community

Felix Koberling, Fabio Barachati, Marcelle Koenig, Maria Loidolt-Krueger, Ellen Schmeyer, Matthias Patting, Marcus Sackrow, Uwe Ortmann, Evangelos Sisamakis, Rainer Erdmann

Proceedings Volume PC12847, PC1284708 (2024) https://doi.org/10.1117/12.3007783

KEYWORDS: Confocal microscopy, Fluorescence microscopy, Fluorescence lifetime imaging, Imaging technologies, Cameras, Video, Time resolved spectroscopy, Time correlated single photon counting, Sampling rates, Picosecond phenomena

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Proceedings Article | 13 March 2024 Presentation

Fast analysis with minimal user interaction in fluorescence lifetime imaging

Felix Koberling, Fabio Barachati, Marcelle König, Maria Loidolt-Krueger, Ellen Schmeyer, Matthias Patting, Marcus Sackrow, Uwe Ortmann, Evangelos Sisamakis, Rainer Erdmann

Proceedings Volume PC12849, PC128490N (2024) https://doi.org/10.1117/12.3001448

KEYWORDS: Fluorescence lifetime imaging, Statistical analysis, Analytical research, Statistical modeling, Process modeling, Multiplexing, Microscopes, Detection and tracking algorithms, Confocal microscopy

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Proceedings Article | 13 March 2024 Poster

Electroluminescence and photoluminescence characterization of LEDs/OLEDs using a single micro-photoluminescence spectroscopy setup

Felix Koberling, Eugeny Ermilov, Christian Oelsner, Bita Rezania, Uwe Ortmann, Mario Gerecke, Rainer Erdmann

Proceedings Volume PC12906, PC129060P (2024) https://doi.org/10.1117/12.3001479

KEYWORDS: Electroluminescence, Spectroscopy, Photoluminescence, Light emitting diodes, Organic light emitting diodes, Electrical properties, Spectroscopes, Spectral resolution, Spatial resolution, Microscopes

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Proceedings Article | 13 March 2024 Poster

Second-harmonic generation imaging microscopy with ps pulsed lasers

Felix Koberling, Volker Buschmann, Christian Oelsner, Bita Rezania, Maria Loidolt-Krüger, Uwe Ortmann, Rainer Erdmann

Proceedings Volume PC12855, PC128551F (2024) https://doi.org/10.1117/12.3001466

KEYWORDS: Second harmonic generation, Biological imaging, Picosecond phenomena, Pulsed laser operation, Microscopes, Crystals, Reflection, Nonlinear crystals, Laser safety, High power lasers

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Proceedings Article | 12 March 2024 Presentation

High resolution event time tagger with ultra short dead-time

Felix Koberling, Tino Roehlicke, Hans-Jürgen Rahn, Nicolai Adelhöfer, Torsten Krause, Torsten Langer, Florian Weigert, Michael Wahl, Rainer Erdmann, Uwe Ortmann

Proceedings Volume PC12893, PC128930D (2024) https://doi.org/10.1117/12.3001488

KEYWORDS: Equipment, Time correlated single photon counting, Quantum channels, Single photon detectors, Quantum optics applications, Fluorescence lifetime imaging, Design and modelling, Visualization, Visual analytics, Superconductors

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Proceedings Article | 17 March 2023 Poster

Fast analysis with minimal user interaction in Fluorescence Lifetime Imaging

Fabio Barachati, Marcelle König, Maria Loidolt-Krüger, Ellen Schmeyer, Matthias Patting, Marcus Sackrow, Uwe Ortmann, Felix Koberling, Rainer Erdmann

Proceedings Volume PC12386, PC123860L (2023) https://doi.org/10.1117/12.2651118

KEYWORDS: Fluorescence lifetime imaging, Statistical modeling, Statistical analysis, Sensors, Process modeling, Multiplexing, Microscopes, Detection and tracking algorithms, Confocal microscopy, Analytical research

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Proceedings Article | 17 March 2023 Poster

Integration of a superconducting nanowire detector into a confocal microscope for TRPL-mapping: sensitivity and time resolution

Volker Buschmann, Eugeny Ermilov, Felix Koberling, Rainer Erdmann, Christian Oelsner, Jürgen Breitlow

Proceedings Volume PC12386, PC123860H (2023) https://doi.org/10.1117/12.2647548

KEYWORDS: Sensors, Nanowires, Superconductors, Microscopes, Confocal microscopy, Quantum efficiency, Luminescence, Single photon detectors, Waveguides, Temporal resolution

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Proceedings Article | 17 March 2023 Poster

Next generation SPAD image sensors and lasers for widefield time domain FLIM

Max Tillmann, Johan Hummert, Felix Koberling, Rainer Erdmann, Cyril Saudan, Harald Homulle, Ivan Michel Antolovic

Proceedings Volume PC12386, PC123860O (2023) https://doi.org/10.1117/12.2666381

KEYWORDS: Fluorescence lifetime imaging, Lasers, Single photon avalanche diodes, Image sensors, Sensors, Time correlated single photon counting, Time resolved spectroscopy, Technology, Statistical analysis, Laser sources

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Proceedings Article | 15 March 2023 Presentation

Easy and reliable way to perform single molecule FRET measurements (Conference Presentation)

Felix Koberling, Marcelle König, Maria Loidolt-Krüger, Fabio Barachati, Matthias Patting, Marcus Sackrow, Kamil Bobowski, Mathias Bayer, Rainer Erdmann

Proceedings Volume PC12386, PC1238603 (2023) https://doi.org/10.1117/12.2649871

KEYWORDS: Molecules, Fluorescence resonance energy transfer, Proteins, Point spread functions, Photonic microstructures, Microscopes, Confocal microscopy

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Proceedings Article | 15 March 2023 Presentation

Small SPAD-arrays for confocal fluoresence lifetime imaging (Conference Presentation)

Max Tillmann, Felix Koberling, Tino Roehlicke, Michael Wahl, Cyril Saudan, Harald Homulle, Ivan Michel Antolovic, Rainer Erdmann

Proceedings Volume PC12384, PC123840G (2023) https://doi.org/10.1117/12.2655332

KEYWORDS: Confocal microscopy, Microscopy, Fluorescence lifetime imaging, Fluorescence correlation spectroscopy, Super resolution, Single photon detectors, Single photon, Signal to noise ratio, Imaging technologies, Data processing

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Proceedings Article | 9 March 2023 Presentation

Advanced data preprocessing features for state-of-the-art TCSPC and time-tagging devices

Torsten Langer, Max Tillmann, Tino Roehlicke, Michael Wahl, Rainer Erdmann, Felix Koberling

Proceedings Volume PC12446, PC1244612 (2023) https://doi.org/10.1117/12.2649759

KEYWORDS: Ranging, LIDAR, Heart, Fluorescent markers, Field programmable gate arrays, Data compression, Analytical research

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Proceedings Article | 7 March 2022 Presentation

Bringing UV excitation of small biomolecules and proteins to another level - pulsed high-power UV-LEDs with more than 40 times higher output power

Christian Oelsner, Linnea Olofsson, Uwe Ortmann, Eugeny Ermilov, Volker Buschmann, Frank Birke, Rainer Erdmann, Mario Gerecke

Proceedings Volume PC11964, PC119640E (2022) https://doi.org/10.1117/12.2605884

KEYWORDS: Proteins, Luminescence, Ultraviolet radiation, Molecules, Time resolved spectroscopy, Spectroscopy, Signal detection, Resonance energy transfer, Picosecond phenomena, Optical testing

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Proceedings Article | 7 March 2022 Presentation

Multi-channel event timer with external FPGA interface

Torsten Langer, Tino Roehlicke, Sebastian Kulisch, Maximilian Diedrich, Michael Wahl, Rainer Erdmann, Mario Gerecke, Uwe Ortmann

Proceedings Volume PC11965, PC119650F (2022) https://doi.org/10.1117/12.2609343

KEYWORDS: Field programmable gate arrays, Single photon, Fluorescence lifetime imaging, Electronics

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Proceedings Article | 7 March 2022 Poster

Non-destructive photoluminescence investigation of PV devices with high spatial resolution microscope techniques

Christian Oelsner, Volker Buschmann, Felix Koberling, Rainer Erdmann, Uwe Ortmann, Linnea Olofsson

Proceedings Volume PC11996, PC119960L (2022) https://doi.org/10.1117/12.2605882

KEYWORDS: Photovoltaics, Luminescence, Spatial resolution, Nondestructive evaluation, Microscopes, Semiconductors, Spectroscopy, Scanning tunneling microscopy, Scanning electron microscopy, Time resolved spectroscopy

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Proceedings Article | 5 March 2022 Presentation

High spatial photoluminescence investigation of nanostructures with single-molecule sensitivity

Uwe Ortmann, Christian Oelsner, Linnea Olofsson, Volker Buschmann, Felix Koberling, Rainer Erdmann

Proceedings Volume PC12010, PC1201015 (2022) https://doi.org/10.1117/12.2605883

KEYWORDS: Luminescence, Nanostructures, Spectroscopy, Raman spectroscopy, Optical properties, Nanoparticles, Time resolved spectroscopy, Quantum efficiency, Quantum dots, Particles

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Proceedings Article | 3 March 2022 Presentation

Measuring steady-state and time-resolved photoluminescence of up-conversion nanoparticles with high spatial resolution

Christian Oelsner, Linnea Olofsson, Uwe Ortmann, Eugeny Ermilov, Volker Buschmann, Matthias Patting, Rainer Erdmann

Proceedings Volume PC11976, PC1197601 (2022) https://doi.org/10.1117/12.2605888

KEYWORDS: Nanoparticles, Spatial resolution, Luminescence, Spectroscopy, Visible radiation, Time resolved spectroscopy, Resonance energy transfer, Optical properties, Near infrared, Molecular interactions

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Proceedings Article | 2 March 2022 Poster

Simultaneous Pulsed Interleaved Excitation (PIE) and Alternating-Laser EXcitation (ALEX): an optimized excitation pattern for smFRET

Kristian Lauritsen, Dietmar Klemme, Guillaume Delpont, Evangelos Sisamakis, Marcelle König, Uwe Ortmann, Felix Koberling, Rainer Erdmann

Proceedings Volume PC11967, PC119670I (2022) https://doi.org/10.1117/12.2622803

Proceedings Article | 5 March 2021 Presentation

Measuring steady-state and time-resolved photoluminescence from a positionable, micrometer-sized observation volume

Christian Oelsner, Eugeny Ermilov, Volker Buschmann, Frank Birke, Matthias Patting, Uwe Ortmann, Rainer Erdmann

Proceedings Volume 11656, 116560H (2021) https://doi.org/10.1117/12.2576400

KEYWORDS: Luminescence, Spectroscopy, Statistical analysis, Molecules, Molecular spectroscopy, Time resolved spectroscopy, Spatial resolution, Semiconductors, Resonance energy transfer, Proteins

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Proceedings Article | 5 March 2021 Presentation

Visualizing dynamic processes with rapidFLIMHiRes, the ultra fast FLIM imaging method with outstanding 10ps time resolution

Maria Loidolt-Krüger, Fabian Jolmes, Matthias Patting, Michael Wahl, Evangelos Sismakis, André Devaux, Uwe Ortmann, Felix Koberling, Rainer Erdmann

Proceedings Volume 11648, 116480D (2021) https://doi.org/10.1117/12.2583477

KEYWORDS: Fluorescence lifetime imaging, Visualization, Ultrafast imaging, Image resolution, Temporal resolution, Sensors, Picosecond phenomena, Fluorescence resonance energy transfer, Tissues, Single photon

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Proceedings Article | 5 March 2021 Presentation

Welcome and Introduction to SPIE Conference: 11650

Rainer Erdmann, Ingo Gregor, Felix Koberling

Proceedings Volume 11650, 1165002 (2021) https://doi.org/10.1117/12.2592756

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Proceedings Article | 5 March 2021 Presentation

Visualizing dynamic processes with rapidFLIMHiRes, the ultra fast FLIM imaging method with outstanding 10ps time resolution

Maria Loidolt-Krüger, Fabian Jolmes, Matthias Patting, Michael Wahl, Evangelos Sismakis, André Devaux, Uwe Ortmann, Felix Koberling, Rainer Erdmann

Proceedings Volume 11654, 116540I (2021) https://doi.org/10.1117/12.2583684

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Proceedings Article | 26 March 2020 Presentation

Scanning FCS and super-resolution microscopy on 2D lipid membranes (Conference Presentation)

Uwe Ortmann, Mariano Gonzalez Pisfil, Marcelle König, Rhys Dowler, Benedikt Krämer, Sumeet Rohilla, Felix Koberling, Rainer Erdmann

Proceedings Volume 11246, 1124607 (2020) https://doi.org/10.1117/12.2542417

KEYWORDS: Fluorescence correlation spectroscopy, Super resolution microscopy, Stimulated emission depletion microscopy, Diffusion, Cell mechanics, Molecules, Calibration, Confocal microscopy, Time resolved spectroscopy, Microscopes

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Proceedings Article | 9 March 2020 Presentation

Multi-target immunofluorescence using spectral FLIM-FRET for separation of undesirable antibody cross-labeling and autofluorescence (Conference Presentation)

Sumeet Rohilla, Benedikt Kraemer, Felix Koberling, Uwe Ortmann, Ingo Gregor, Andreas Hocke, Rainer Erdmann

Proceedings Volume 11244, 1124418 (2020) https://doi.org/10.1117/12.2542413

KEYWORDS: Molecules, Fluorescence resonance energy transfer, Fluorescence lifetime imaging, Molecular interactions, Luminescence, Electronics, Data analysis, Time resolved spectroscopy, Lung, Tissues

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Proceedings Article | 21 February 2020 Paper

Programmable, pulse shaped diode laser

Paul Fey, Thomas Schönau, Kristian Lauritsen, Rainer Erdmann

Proceedings Volume 11259, 112591X (2020) https://doi.org/10.1117/12.2542421

KEYWORDS: Semiconductor lasers, Picosecond phenomena, Pulse shaping, Clocks, Modulation, Analog electronics, Logic

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SPIE Journal Paper | 10 May 2019 Open Access

BabyLux device: a diffuse optical system integrating diffuse correlation spectroscopy and time-resolved near-infrared spectroscopy for the neuromonitoring of the premature newborn brain

Martina Giovannella, Davide Contini, Marco Pagliazzi, Antonio Pifferi, Lorenzo Spinelli, Rainer Erdmann, Roger Donat, Ignacio Rocchetti, Matthias Rehberger, Niels König, Robert Schmitt, Alessandro Torricelli, Turgut Durduran, Udo Weigel

NPh, Vol. 6, Issue 02, 025007, (May 2019) https://doi.org/10.1117/12.10.1117/1.NPh.6.2.025007

KEYWORDS: Optical properties, Near infrared spectroscopy, Sensors, Solids, Diffusion, Tissues, Spectroscopes, Liquids, Tissue optics, Neurophotonics

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Proceedings Article | 4 March 2019 Presentation

Spectrally resolved and high speed TCSPC-based fluorescence lifetime imaging (Conference Presentation)

Tino Roehlicke, Matthias Patting, Hans-Juergen Rahn, Benedikt Kraemer, Michael Wahl, Felix Koberling, Rainer Erdmann

Proceedings Volume 10889, 108890Q (2019) https://doi.org/10.1117/12.2513895

KEYWORDS: Fluorescence lifetime imaging, Signal detection, Sensors, Live cell imaging, Single photon, Luminescence, Electronics, Image acquisition, Picosecond phenomena, Confocal microscopy

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Proceedings Article | 4 March 2019 Presentation

Mapping molecules quantitatively in confocal fluorescence microscopy (Conference Presentation)

Marcelle Koenig, Caroline Berlage, Paja Reisch, Felix Koberling, Uwe Ortmann, Haisen Ta, Rainer Erdmann

Proceedings Volume 10884, 1088409 (2019) https://doi.org/10.1117/12.2507391

KEYWORDS: Confocal microscopy, Luminescence, Microscopy, Molecules, Statistical analysis, Associative arrays, Single photon, Systems modeling, Tantalum, Molecular lasers

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Proceedings Article | 4 March 2019 Presentation

Quantitative ultra-fast FLIM and lifetime based multi-species analysis (Conference Presentation)

Benedikt Kraemer, Sumeet Rohilla, Rhys Dowler, Marcus Sackrow, Matthias Patting, Hans-Jürgen Rahn, Michael Wahl, Uwe Ortmann, Felix Koberling, Andreas Hocke, Rainer Erdmann

Proceedings Volume 10882, 108820K (2019) https://doi.org/10.1117/12.2507396

KEYWORDS: Fluorescence lifetime imaging, Ultrafast phenomena, Tissues, Image processing, Luminescence, Confocal microscopy, Life sciences, Photomultipliers, Sensors, Spatial resolution

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Proceedings Article | 24 April 2017 Presentation

Rapid FLIM: The new and innovative method for ultra-fast imaging of biological processes (Conference Presentation)

Sandra Orthaus-Mueller, Benedikt Kraemer, Astrid Tannert, Tino Roehlicke, Michael Wahl, Hans-Juergen Rahn, Felix Koberling, Rainer Erdmann

Proceedings Volume 10069, 1006919 (2017) https://doi.org/10.1117/12.2249757

KEYWORDS: Ultrafast phenomena, Data acquisition, Sensors, Photons, Fluorescence lifetime imaging, Signal processing, Single photon, Fluorescence resonance energy transfer, Image processing, Time resolved spectroscopy

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Proceedings Article | 20 February 2017 Paper

Picosecond pulsed micro-module emitting near 560 nm using a frequency doubled gain-switched DBR ridge waveguide semiconductor laser

André Kaltenbach, Julian Hofmann, Dirk Seidel, Kristian Lauritsen, Frank Bugge, Jörg Fricke, Katrin Paschke, Rainer Erdmann, Günther Tränkle

Proceedings Volume 10088, 1008808 (2017) https://doi.org/10.1117/12.2250145

KEYWORDS: Picosecond phenomena, Semiconductor lasers, Waveguides, Second-harmonic generation, Waveguide lasers, Distributed Bragg reflectors, Harmonic generation, Fluorescence spectroscopy, Pulsed laser operation, Picosecond lasers, Nonlinear crystals, Laser sources, Frequency conversion

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Proceedings Article | 14 March 2016 Paper

ns-time resolution for multispecies STED-FLIM and artifact free STED-FCS

Marcelle Koenig, Paja Reisch, Rhys Dowler, Benedikt Kraemer, Sebastian Tannert, Matthias Patting, Mathias Clausen, Silvia Galiani, Christian Eggeling, Felix Koberling, Rainer Erdmann

Proceedings Volume 9712, 97120T (2016) https://doi.org/10.1117/12.2208874

KEYWORDS: Fluorescence correlation spectroscopy, Super resolution, Microscopy, Pulsed laser operation, Luminescence, Picosecond phenomena, Stimulated emission depletion microscopy, Optical resolution, Time correlated photon counting, Single photon, Diffusion, Confocal microscopy, Molecules, Diffraction

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Proceedings Article | 27 February 2015 Paper

Generation of third harmonic picosecond pulses at 355 nm by sum frequency mixing in periodically poled MgSLT crystal

André Kaltenbach, Thomas Schönau, Kristian Lauritsen, Günther Tränkle, Rainer Erdmann

Proceedings Volume 9347, 93471O (2015) https://doi.org/10.1117/12.2077169

KEYWORDS: Crystals, Nonlinear crystals, Picosecond phenomena, Semiconductor lasers, Harmonic generation, Frequency conversion, Ultraviolet radiation, Laser systems engineering, Fiber amplifiers, Magnesium

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Proceedings Article | 12 March 2013 Paper

Pulsed picosecond 766 nm laser source operating between 1-80 MHz with automatic pump power management

Thomas Schönau, Torsten Siebert, Romano Härtel, Thomas Eckhardt, Dietmar Klemme, Kristian Lauritsen, Rainer Erdmann

Proceedings Volume 8604, 860409 (2013) https://doi.org/10.1117/12.2003900

KEYWORDS: Picosecond phenomena, Semiconductor lasers, Fiber amplifiers, Crystals, Optical amplifiers, Laser sources, Second-harmonic generation, Frequency conversion, Lithium niobate, Stimulated emission depletion microscopy

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Proceedings Article | 26 February 2013 Paper

Picosecond supercontinuum laser with consistent emission parameters over variable repetition rates from 1 to 40 MHz

Thomas Schönau, Torsten Siebert, Romano Härtel, Dietmar Klemme, Kristian Lauritsen, Rainer Erdmann

Proceedings Volume 8601, 86012L (2013) https://doi.org/10.1117/12.2003859

KEYWORDS: Picosecond phenomena, Visible radiation, Fiber amplifiers, Optical amplifiers, Semiconductor lasers, Diodes, Microcontrollers, Phase modulation, Luminescence, Spectroscopy

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Proceedings Article | 10 October 2012 Paper

Characterization and quality control of semiconductor wafers using time-correlated single photon counting

V. Buschmann, S. Fore, F. Koberling, A. Knigge, P. Kapusta, U. Ortmann, M. Roczen, R. Erdmann

Proceedings Volume 8470, 84700F (2012) https://doi.org/10.1117/12.929814

KEYWORDS: Luminescence, Sensors, Quantum wells, Semiconducting wafers, Single photon, Picosecond phenomena, Gallium arsenide, Pulsed laser operation, Semiconductor materials, Semiconductor lasers

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Proceedings Article | 15 February 2012 Paper

Picosecond pulse generation at 596 nm via sum-frequency mixing with a gain-switched, fiber-amplified laser diode

Kristian Lauritsen, Thomas Schönau, Torsten Siebert, Rainer Erdmann

Proceedings Volume 8240, 824003 (2012) https://doi.org/10.1117/12.906408

KEYWORDS: Diodes, Picosecond phenomena, Semiconductor lasers, Optical amplifiers, Waveguides, Sum-frequency generation, Polarization, Wavelength division multiplexing, Ferroelectric materials, Laser sources

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Proceedings Article | 3 February 2012 Paper

Time-resolved single molecule microscopy coupled with atomic force microscopy

M. König, F. Koberling, O. Schulz, R. Ros, S. Fore, M. Sackrow, S. Trautmann, R. Erdmann

Proceedings Volume 8227, 822719 (2012) https://doi.org/10.1117/12.906177

KEYWORDS: Atomic force microscopy, Luminescence, Confocal microscopy, Molecules, Microscopes, Fluorescence lifetime imaging, Microscopy, Data acquisition, Imaging systems, Green fluorescent protein

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Proceedings Article | 14 May 2011 Paper

Tau-SPAD: a new red sensitive single-photon counting module

Gerald Kell, Andreas Bülter, Michael Wahl, Rainer Erdmann

Proceedings Volume 8033, 803303 (2011) https://doi.org/10.1117/12.884754

KEYWORDS: Sensors, Single photon, Picosecond phenomena, Electronics, Photodetectors, Avalanche photodiodes, Molecules, Fluorescence correlation spectroscopy, Photon counting, Quenching (fluorescence)

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Proceedings Article | 23 February 2011 Paper

Buffer controlled photoswitching microscopy using standard organic fluorophores

Volker Buschmann, Samantha Fore, Sebastian van de Linde, Markus Sauer, Steve Wolter, Mike Heilemann, Felix Koberling, Rainer Erdmann

Proceedings Volume 7905, 790510 (2011) https://doi.org/10.1117/12.873383

KEYWORDS: Molecules, Luminescence, Microscopy, Cameras, Super resolution microscopy, Image quality, Super resolution, Ions, Switches, Microscopes

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Proceedings Article | 22 February 2011 Paper

Amplification of ps-pulses from freely triggerable gain-switched laser diodes at 1062 nm and second harmonic generation in periodically poled lithium niobate

Thomas Schönau, Sina Riecke, Kristian Lauritsen, Rainer Erdmann

Proceedings Volume 7917, 791707 (2011) https://doi.org/10.1117/12.872114

KEYWORDS: Picosecond phenomena, Semiconductor lasers, Fiber amplifiers, Second-harmonic generation, Harmonic generation, Crystals, Optical amplifiers, Lithium niobate, Luminescence, Laser crystals

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Proceedings Article | 11 February 2011 Paper

Fast algorithms for the analysis of spectral FLIM data

I. Gregor, B. Krämer, F. Koberling, R. Erdmann, J. Enderlein, M. Wahl, S. Fore

Proceedings Volume 7903, 790330 (2011) https://doi.org/10.1117/12.873386

KEYWORDS: Fluorescence lifetime imaging, Luminescence, Fluorescence resonance energy transfer, Signal detection, Data conversion, Single photon, Picosecond phenomena, Microscopes, Statistical analysis, Fluorescence spectroscopy

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Proceedings Article | 26 February 2010 Paper

Fluorescence performance standards for confocal microscopy

Steffen Rüttinger, Peter Kapusta, Volker Völlkopf, Felix Koberling, Rainer Erdmann, Rainer Macdonald

Proceedings Volume 7569, 75692I (2010) https://doi.org/10.1117/12.840501

KEYWORDS: Luminescence, Confocal microscopy, Molecules, Microscopes, Glasses, Spatial resolution, Diffusion, Standards development, Fluorescence correlation spectroscopy, Fluorescence spectroscopy

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Proceedings Article | 26 February 2010 Paper

Fast rasterscanning enables FLIM in macroscopic samples up to several centimeters

F. Koberling, V. Buschmann, C. Hille, M. Patting, C. Dosche, A. Sandberg, A. Wheelock, R. Erdmann

Proceedings Volume 7569, 756931 (2010) https://doi.org/10.1117/12.843453

KEYWORDS: Fluorescence lifetime imaging, Luminescence, Scanners, Proteins, Microscopes, Single photon, Sensors, Laser scanners, Multiplexing, Imaging systems

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Proceedings Article | 25 February 2010 Paper

The use of time-resolved fluorescence in gel-based proteomics for improved biomarker discovery

AnnSofi Sandberg, Volker Buschmann, Peter Kapusta, Rainer Erdmann, Åsa Wheelock

Proceedings Volume 7568, 756811 (2010) https://doi.org/10.1117/12.842571

KEYWORDS: Proteins, Luminescence, Medicine, Image acquisition, Time resolved spectroscopy, Laser scanners, Photon counting, Fluorescence spectroscopy, Tissues, Visualization

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Proceedings Article | 25 February 2009 Paper

New multichannel photon timing instrumentation with independent synchronized channels and high count rate for FLIM and correlation analysis

Michael Wahl, Gerald Kell, Peter Kapusta, Hans-Jürgen Rahn, Tino Roehlicke, Rainer Erdmann

Proceedings Volume 7183, 71830C (2009) https://doi.org/10.1117/12.808716

KEYWORDS: Sensors, Luminescence, Fluorescence lifetime imaging, Molecules, Fluorescence correlation spectroscopy, Microchannel plates, Picosecond phenomena, Signal detection, Photodetectors, Electronics

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Proceedings Article | 25 February 2009 Paper

Simultaneous monitoring of the two coupled motors of a single FoF1-ATP synthase by three-color FRET using duty cycle-optimized triple-ALEX

N. Zarrabi, S. Ernst, M. Düser, A. Golovina-Leiker, W. Becker, R. Erdmann, S. Dunn, M. Börsch

Proceedings Volume 7185, 718505 (2009) https://doi.org/10.1117/12.809610

KEYWORDS: Fluorescence resonance energy transfer, Acquisition tracking and pointing, Luminescence, Pulsed laser operation, Picosecond phenomena, Proteins, Confocal microscopy, Avalanche photodetectors, Bragg cells, Optical filters

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Proceedings Article | 25 February 2009 Paper

Recent advances in photon coincidence measurements for photon antibunching and full correlation analysis

F. Koberling, B. Kraemer, V. Buschmann, S. Ruettinger, P. Kapusta, M. Patting, M. Wahl, R. Erdmann

Proceedings Volume 7185, 71850Q (2009) https://doi.org/10.1117/12.807803

KEYWORDS: Molecules, Signal detection, Sensors, Picosecond phenomena, Luminescence, Fluorescence correlation spectroscopy, Diffusion, Confocal microscopy, Electronics, Time metrology

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Proceedings Article | 13 February 2009 Paper

Fluorescent nanodiamonds for FRET-based monitoring of a single biological nanomotor FoF1-ATP synthase

M. Börsch, R. Reuter, G. Balasubramanian, R. Erdmann, F. Jelezko, J. Wrachtrup

Proceedings Volume 7183, 71832N (2009) https://doi.org/10.1117/12.812720

KEYWORDS: Fluorescence resonance energy transfer, Diamond, Acquisition tracking and pointing, Luminescence, Nanoparticles, Nanocrystals, Proteins, Nitrogen, Chemical species, Color centers

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Proceedings Article | 7 February 2009 Paper

Comparison of a Yb-doped fiber and a semiconductor taper for amplification of picosecond laser pulses

Sina Riecke, Kristian Lauritsen, Hendrick Thiem, Katrin Paschke, Rainer Erdmann

Proceedings Volume 7212, 72120O (2009) https://doi.org/10.1117/12.808688

KEYWORDS: Fiber amplifiers, Optical amplifiers, Semiconductors, Semiconductor optical amplifiers, Picosecond phenomena, Semiconductor lasers, Oscillators, Amplifiers, Optical isolators, Indium gallium arsenide

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Proceedings Article | 21 February 2008 Paper

Recent advances in time-correlated single-photon counting

Felix Koberling, Benedikt Krämer, Sebastian Tannert, Steffen Rüttinger, Uwe Ortmann, Matthias Patting, Michael Wahl, Benjamin Ewers, Peter Kapusta, Rainer Erdmann

Proceedings Volume 6862, 686209 (2008) https://doi.org/10.1117/12.761161

KEYWORDS: Fluorescence correlation spectroscopy, Photons, Luminescence, Sensors, Diffusion, Pulsed laser operation, Picosecond phenomena, Confocal microscopy, Molecules, Light scattering

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Proceedings Article | 21 February 2008 Paper

Latest applications for 2-focus fluorescence correlation spectroscopy

T. Dertinger, I. von der Hocht, A. Loman, R. Erdmann, J. Enderlein

Proceedings Volume 6862, 686202 (2008) https://doi.org/10.1117/12.762365

KEYWORDS: Fluorescence correlation spectroscopy, Diffusion, Refractive index, Calcium, Molecules, Luminescence, Prisms, Glasses, Proteins, Pulsed laser operation

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Proceedings Article | 20 February 2008 Paper

Fiber amplified and frequency doubled diode lasers as a highly flexible pulse source at 532nm

Kristian Lauritsen, Sina Riecke, Martin Langkopf, Dietmar Klemme, Christopher Kaleva, Christopher Pallassis, Shirley McNeil, Rainer Erdmann

Proceedings Volume 6871, 68711L (2008) https://doi.org/10.1117/12.762906

KEYWORDS: Semiconductor lasers, Fiber amplifiers, Picosecond phenomena, Solid state lasers, Laser applications, Crystals, Laser crystals, Phase modulation, Modulation, Second-harmonic generation

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Proceedings Article | 15 February 2008 Paper

FLIM and FCS measurements performed with a master oscillator fiber amplifier (MOFA) laser at 530 nm

Felix Koberling, Martin Langkopf, Dietmar Klemme, Andreas Bülter, Volker Buschmann, Kristian Lauritsen, Rainer Erdmann

Proceedings Volume 6860, 68601N (2008) https://doi.org/10.1117/12.761229

KEYWORDS: Semiconductor lasers, Luminescence, Fluorescence lifetime imaging, Fluorescence correlation spectroscopy, Fiber amplifiers, Confocal microscopy, Microscopes, Fiber lasers, Pulsed laser operation, Picosecond phenomena

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Proceedings Article | 15 February 2008 Paper

Advanced FRET and FCS measurements with laser scanning microscopes based on time-resolved techniques

B. Krämer, V. Buschmann, U. Ortmann, F. Koberling, M. Wahl, M. Patting, P. Kapusta, A. Bülter, R. Erdmann

Proceedings Volume 6860, 68601D (2008) https://doi.org/10.1117/12.761141

KEYWORDS: Sensors, Luminescence, Fluorescence correlation spectroscopy, Fluorescence resonance energy transfer, Pulsed laser operation, Laser scanners, Microscopes, Single photon, Data acquisition, Fluorescence lifetime imaging

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Proceedings Article | 11 May 2007 Paper

Advanced TCSPC electronics and their use in general quantum physics and demanding analytical applications

Michael Wahl, Hans-Juergen Rahn, Matthias Patting, Felix Koberling, Rainer Erdmann

Proceedings Volume 6583, 658306 (2007) https://doi.org/10.1117/12.722799

KEYWORDS: Photons, Luminescence, Picosecond phenomena, Molecules, Electronics, Sensors, Time metrology, Fluorescence correlation spectroscopy, Fluorescence lifetime imaging, Quantum physics

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Proceedings Article | 11 May 2007 Paper

Time-resolved confocal fluorescence microscopy: novel technical features and applications for FLIM, FRET and FCS using a sophisticated data acquisition concept in TCSPC

Felix Koberling, Benedikt Krämer, Peter Kapusta, Matthias Patting, Michael Wahl, Rainer Erdmann

Proceedings Volume 6583, 65830Y (2007) https://doi.org/10.1117/12.722867

KEYWORDS: Fluorescence resonance energy transfer, Luminescence, Molecules, Fluorescence correlation spectroscopy, Confocal microscopy, Fluorescence lifetime imaging, Data acquisition, Molecular photonics, Microscopy, Picosecond phenomena

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In recent years time-resolved fluorescence measurement and analysis techniques became a standard in single molecule microscopy. However, considering the equipment and experimental implementation they are typically still an add-on and offer only limited possibilities to study the mutual dependencies with common intensity and spectral information. In contrast, we are using a specially designed instrument with an unrestricted photon data acquisition approach which allows to store spatial, temporal, spectral and intensity information in a generalized format preserving the full experimental information. This format allows us not only to easily study dependencies between various fluorescence parameters but also to use, for example, the photon arrival time for sorting and weighting the detected photons to improve the significance in common FCS and FRET analysis schemes. The power of this approach will be demonstrated for different techniques: In FCS experiments the concentration determination accuracy can be easily improved by a simple time-gated photon analysis to suppress the fast decaying background signal. A more detailed analysis of the arrival times allows even to separate FCS curves for species which differ in their fluorescence lifetime but, for example, cannot be distinguished spectrally. In multichromophoric systems like a photonic wire which undergoes unidirectional multistep FRET the lifetime information complements significantly the intensity based analysis and helps to assign the respective FRET partners. Moreover, together with pulsed excitation the time-correlated analysis enables directly to take advantage of alternating multi-colour laser excitation. This pulsed interleaved excitation (PIE) can be used to identify and rule out inactive FRET molecules which cause interfering artefacts in standard FRET efficiency analysis. We used a piezo scanner based confocal microscope with compact picosecond diode lasers as excitation sources. The timing performance can be significantly increased by using new SPAD detectors which enable, in conjunction with new TCSPC electronics, an overall IRF width of less than 120 ps maintaining single molecule sensitivity.

Proceedings Article | 11 May 2007 Paper

Dead-time effects in TCSPC data analysis

Matthias Patting, Michael Wahl, Peter Kapusta, Rainer Erdmann

Proceedings Volume 6583, 658307 (2007) https://doi.org/10.1117/12.722804

KEYWORDS: Data modeling, Photodetectors, Picosecond phenomena, Sensors, Data analysis, Time correlated photon counting, Error analysis, Luminescence, Single photon, Monochromators

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Proceedings Article | 27 February 2006 Paper

Measuring precise diffusion coefficients with two-focus fluorescence correlation spectroscopy

Thomas Dertinger, Ingo Gregor, Iris von der Hocht, Rainer Erdmann, Benedikt Krämer, Felix Koberling, Rudolf Hartmann, Jörg Enderlein

Proceedings Volume 6092, 609203 (2006) https://doi.org/10.1117/12.651053

KEYWORDS: Diffusion, Fluorescence correlation spectroscopy, Luminescence, Molecules, Pulsed laser operation, Polarization, Beam splitters, Objectives, Photodetectors, Data modeling

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Proceedings Article | 27 February 2006 Paper

Identification of triplet state and photoisomerization of Cy5 by means of ensemble and single-molecule measurements

Zhengxi Huang, Dongmei Ji, Sufan Wang, Andong Xia, Felix Koberling, Matthias Patting, Rainer Erdmann

Proceedings Volume 6092, 60920Y (2006) https://doi.org/10.1117/12.650492

KEYWORDS: Molecules, Luminescence, Absorption, Phosphorescence, Bioalcohols, Molecular lasers, Photolysis, Chemical species, Glasses, Spectrophotometry

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Proceedings Article | 28 April 2005 Paper

Amplified picosecond diode lasers for diffuse optical imaging and spectroscopy of tissue

R. Erdmann, M. Langkopf, K. Lauritsen, A. Bulter, M. Wahl, H. Wabnitz, A. Liebert, M. Moller, T. Schmitt

Proceedings Volume 5693, (2005) https://doi.org/10.1117/12.590419

KEYWORDS: Semiconductor lasers, Diffuse optical imaging, Optical spectroscopy, Picosecond phenomena, Spectroscopy, Imaging spectroscopy, Optical spectroscopy imaging, Tissue optics, Laser tissue interaction, Optical tomography

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Proceedings Article | 27 April 2005 Paper

10-W peak power from a gain-switched picosecond all-semiconductor laser

Reto Haring, Thorsten Schmitt, Aude-Reine Bellancourt, Frank Lison, Kristian Lauritsen, Rainer Erdmann, Wilhelm Kaenders

Proceedings Volume 5707, (2005) https://doi.org/10.1117/12.591479

KEYWORDS: Semiconductor lasers, Picosecond phenomena, Optical amplifiers, Laser systems engineering, Microscopy, Pulsed laser operation, Solids, Second-harmonic generation, Diodes, Oscillators

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Proceedings Article | 30 March 2005 Paper

Time-resolved laser scanning microscopy with FLIM and advanced FCS capability

Benedict Kraemer, Felix Koberling, Uwe Ortmann, Michael Wahl, Peter Kapusta, Andreas Buelter, Rainer Erdmann

Proceedings Volume 5700, (2005) https://doi.org/10.1117/12.590500

KEYWORDS: Laser scanners, Data acquisition, Microscopes, Picosecond phenomena, Sensors, Luminescence, Fluorescence lifetime imaging, Fluorescence correlation spectroscopy, Semiconductor lasers, Microscopy

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We present the technical integration of state-of-the-art picosecond diode laser sources and data acquisition electronics in conventional laser scanning microscopes. This procedure offers users of laser scanning microscopes an easy upgrade path towards time-resolved measurements. Our setup uses picosecond diode lasers from 375 to 800 nm for excitation which are coupled to the microscope via a single mode fiber. The corresponding emission is guided to a fibre coupled photon counting detector, such as Photomultiplier Tubes (PMT) or Single Photon Avalanche Diodes (SPAD). This combines the outstanding sensitivity of photon counting detectors with the ease of use of diode laser sources, to allow time-resolved measurements of fluorescence decays with resolutions down to picoseconds. The synchronization signals from the laser scanning microscope are fed into the data stream recorded by the TimeHarp 200 TCSPC system, via the unique Time-Tagged Time-Resolved (TTTR) data acquisition mode. In this TTTR data acquisition mode each photon is recorded individually with its specific parameters as detector channel, picosecond timing, global arrival time and, in this special application, up to three additional markers. These markers, in combination with the global arrival time, allow the system software to reconstruct the complete image and subsequently create the full fluorescence lifetime image (FLIM). The multi-parameter data acquisition scheme of the TimeHarp 200 electronics not only records each parameter individually, but offers in addition the opportunity to analyse the parameter dependencies in a multitude of different ways. This method allows for example to calculate the fluorescence fluctuation correlation function (FCS) on any single spot of interest but also to reconstruct the fluorescence decay of each image pixel and detector channel for advanced Forster Resonance Energy Transfer (FRET) analysis. In this paper, we present some selected results acquired with standard laser scanning microscopes upgraded towards temporal resolution.

Proceedings Article | 29 March 2005 Paper

Advanced multifocus confocal laser scanning microscope for single molecule studies

Thomas Dertinger, Felix Koberling, Ales Benda, Rainer Erdmann, Martin Hof, Joerg Enderlein

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.611147

KEYWORDS: Molecules, Luminescence, Confocal microscopy, Diffusion, Fluorescence correlation spectroscopy, Pulsed laser operation, Particles, Microscopes, Fluorescence spectroscopy, Photodetectors

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Proceedings Article | 18 June 2004 Paper

Compact TCSPC upgrade package for laser scanning microscopes based on 375- to 470-nm picosecond diode lasers

Uwe Ortmann, T. Dertinger, Michael Wahl, Matthias Patting, Rainer Erdmann

Proceedings Volume 5325, (2004) https://doi.org/10.1117/12.536888

KEYWORDS: Laser scanners, Luminescence, Sensors, Semiconductor lasers, Picosecond phenomena, Data acquisition, Microscopes, Photon counting, Signal detection, Fluorescence lifetime imaging

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We present the technical integration of state-of-the-art picosecond diode laser sources and data acquisition electronics in conventional laser scanning microscopes. This offers users of laser scanning microscopes an easy upgrade path towards time-resolved measurements. Our setup uses picosecond diode lasers from 375 nm, 405 nm, 440 nm and 470 nm for fluorescence excitation which are coupled in through a sole single mode fiber. The detected signal is guided to a photon counting detector, such as Photomultiplier Tubes (PMT) or Single Photon Avalanche Diodes (SPAD). This combines the outstanding sensitivity of photon counting detectors with the ease of use of diode laser sources, to allow time-resolved measurements of fluorescence decays with resolutions down to picoseconds. The synchronization signals from the laser scanning microscope are fed into the data stream recorded by the TimeHarp 200 TCSPC5,7 system, via the unique Time-Tagged Time-Resolved (TTTR)6 data acquisition mode. In this TTTR data acquisition mode each photon is recorded individually with its specific parameters as detector channel, picosecond timing, global arrival time and, in this special application, up to three additional markers. These markers, in combination with the global arrival time, allow the system software to reconstruct the complete image and subsequently fit the full fluorescence lifetime image. The multi-parameter data acquisition scheme of the TimeHarp 200 electronics not only records each parameter individually, but offers in addition the opportunity to analyse the parameter dependencies in a multitude of different ways. This method allows not only to calculate the fluorescence fluctuation correlation function (FCS) on any single spot of interest but also to reconstruct the fluorescence decay of each image pixel and detector channel for the purpose of Fluorescence Lifetime Imaging (FLIM) or advanced Fluorescence Resonance Energy Transfer (FRET) analysis. We present here some selected results acquired with standard laser scanning microscopes upgraded for the time-correlated single photon counting technique.

Proceedings Article | 14 October 2003 Paper

Two-channel fluorescence lifetime microscope with two colour laser excitation, single-molecule sensitivity, and submicrometer resolution

Felix Koberling, Michael Wahl, Matthias Patting, Hans-Juergen Rahn, Peter Kapusta, Rainer Erdmann

Proceedings Volume 5143, (2003) https://doi.org/10.1117/12.500610

KEYWORDS: Luminescence, Molecules, Microscopes, Fluorescence lifetime imaging, Sensors, Fluorescence correlation spectroscopy, Polarization, Picosecond phenomena, Single photon, Electronics

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SPIE Journal Paper | 1 January 2003 Open Access

Time-resolved microspectrofluorometry and fluorescence lifetime imaging of photosensitizers using picosecond pulsed diode lasers in laser scanning microscopes

Matthias Kress, Thomas Meier, Rudolf Steiner, Frank Dolp, Rainer Erdmann, Uwe Ortmann, Angelika Rueck

JBO, Vol. 8, Issue 01, (January 2003) https://doi.org/10.1117/12.10.1117/1.1528595

Read Abstract +

This work describes the time-resolved fluorescence characteristics of two different photosensitizers in single cells, in detail mTHPC and 5-ALA induced PPIX, which are currently clinically used in photodynamic therapy. The fluorescence lifetime of the drugs was determined in the cells from time-gated spectra as well as single photon counting, using a picosecond pulsed diode laser for fluorescence excitation. The diode laser, which emits pulses at 398 nm with 70 ps full width at half maximum duration, was coupled to a confocal laser scanning microscope. For time-resolved spectroscopy a setup consisting of a Czerny Turner spectrometer and a MCP-gated and -intensified CCD camera was used. Time-gated spectra within the cells were acquired by placing the laser beam in ‘‘spot scan’’ mode. In addition, a time-correlated single photon counting module was used to determine the fluorescence lifetime from single spots and to record lifetime images. The fluorescence lifetime of mTHPC decreased from 7.5 to 5.5 ns during incubation from 1 to 6 h. This decrease was probably attributed to enhanced formation of aggregates during incubation. Fluorescence lifetime imaging showed that longer lifetimes were correlated with accumulation in the cytoplasm in the neighborhood of the cell nucleus, whereas shorter lifetimes were found in the outer cytoplasm. For cells that were incubated with 5-ALA, a fluorescence lifetime of 7.4 ns was found for PPIX; a shorter lifetime at 3.6 ns was probably attributed to photoproducts and aggregates of PPIX. In contrast from fluorescence intensity images alone, different fluorescence species could not be distinguished. However, in the lifetime image a structured fluorescence distribution in the cytoplasm was correlated with the longer lifetime and probably coincides with mitochondria. In conclusion, picosecond diode lasers coupled to a laser scanning microscope equipped with appropriate detection units allows time-resolved spectroscopy and lifetime imaging with high spatial resolution and provides numerous possibilities in cellular and pharmaceutical research.

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Proceedings Article | 16 April 2002 Paper

Application of sub-ns pulsed LEDs in fluorescence lifetime spectroscopy

Michael Wahl, Uwe Ortmann, Kristian Lauritsen, Rainer Erdmann

Proceedings Volume 4648, (2002) https://doi.org/10.1117/12.462651

KEYWORDS: Luminescence, Light emitting diodes, Picosecond phenomena, Semiconductor lasers, Molecules, Single photon, Electronics, Light sources, Fluorescence spectroscopy, Spectroscopy

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Proceedings Article | 28 March 2002 Paper

Fluorescence lifetime imaging system with nm-resolution and single-molecule sensitivity

Michael Wahl, Hans-Juergen Rahn, Uwe Ortmann, Rainer Erdmann, Martin Boehmer, Joerg Enderlein

Proceedings Volume 4634, (2002) https://doi.org/10.1117/12.463829

KEYWORDS: Molecules, Luminescence, Picosecond phenomena, Scanners, Semiconductor lasers, Fluorescence lifetime imaging, Single photon, Clocks, Sensors, Electronics

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Proceedings Article | 3 May 1999 Paper

Simple scanning near-infrared time-correlated single-photon counting instrument with a pulsed diode laser and avalanche photodiode (APD) for time-resolved measurements in scanning applications with

Yuling Zhang, Steven Soper, Lyle Middendorf, John Wrum, Rainer Erdmann, Michael Wahl

Proceedings Volume 3602, (1999) https://doi.org/10.1117/12.347539

KEYWORDS: Avalanche photodetectors, Single photon, Microscopes, Semiconductor lasers, Luminescence, Head, Avalanche photodiodes, Picosecond phenomena, Aluminum, Sensors

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Proceedings Article | 10 April 1998 Paper

Hardware solution for continuous time-resolved burst detection of single molecules in flow

Michael Wahl, Rainer Erdmann, Kristian Lauritsen, Hans-Juergen Rahn

Proceedings Volume 3259, (1998) https://doi.org/10.1117/12.307325

KEYWORDS: Luminescence, Molecules, Field programmable gate arrays, Picosecond phenomena, Single photon, Optical resolution, Semiconductor lasers, Photon counting, Time metrology, Liquids

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Proceedings Article | 22 May 1997 Paper

All-solid-state TCPC instrument for dynamic lifetime measurements in sensitive DNA analysis

Rainer Erdmann, Michael Wahl, Kristian Lauritsen, Joerg Enderlein, Benjamin Legendre, Daryl Williams, Steven Soper

Proceedings Volume 2985, (1997) https://doi.org/10.1117/12.274364

KEYWORDS: Picosecond phenomena, Semiconductor lasers, Luminescence, Single photon, Capillaries, Solid state electronics, Solid state lasers, Electronics, Light scattering, Electro optical modeling

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Proceedings Article | 1 April 1996 Paper

High-speed electronics for the detection of time-resolved fluorescence in a continuous flow system

Rainer Erdmann, Uwe Ortmann, Joerg Enderlein, Wolfgang Becker, Michael Wahl, Edgar Klose

Proceedings Volume 2680, (1996) https://doi.org/10.1117/12.237635

KEYWORDS: Molecules, Luminescence, Photons, Picosecond phenomena, High speed electronics, Fluorescence spectroscopy, Time resolved spectroscopy, Molecular lasers, Laser induced fluorescence, Clocks

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Proceedings Article | 1 April 1996 Paper

High-repetion-rate picosecond diode lasers for potential use in single-molecule detection

Uwe Ortmann, Rolf Krahl, Gerald Kell, Rainer Erdmann, Joerg Enderlein, Edgar Klose

Proceedings Volume 2680, (1996) https://doi.org/10.1117/12.237636

KEYWORDS: Semiconductor lasers, Molecules, Picosecond phenomena, Fluorescence spectroscopy, Molecular lasers, Laser systems engineering, Electronics, Liquids, Single photon, Lasers

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Proceedings Article | 8 May 1995 Paper

Electron transfer dyes as standard for picosecond fluorescence decay measurements

Jens Sobek, Arno Wiehe, Joerg Enderlein, Rainer Erdmann, Harry Kurreck

Proceedings Volume 2388, (1995) https://doi.org/10.1117/12.208495

KEYWORDS: Luminescence, Picosecond phenomena, Absorption, Zinc, Systems modeling, Antimony, Quantum efficiency, Electroluminescence, Catalysis, Oxidation

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Proceedings Article | 8 May 1995 Paper

Simultaneous detection of time-resolved emission spectra using a multianode PMT and new time-correlated single-photon counting (TCSPC) electronics with a 5-MHz count rate

Rainer Erdmann, Wolfgang Becker, Uwe Ortmann, Joerg Enderlein

Proceedings Volume 2388, (1995) https://doi.org/10.1117/12.208493

KEYWORDS: Picosecond phenomena, Sensors, Electronics, Luminescence, Single photon, Ultrafast phenomena, Monochromators, Time metrology, Oscilloscopes, Signal detection

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Proceedings Article | 8 May 1995 Paper

Lifetime-based identification of single molecules

Joerg Enderlein, Rolf Krahl, Uwe Ortmann, Michael Wahl, Rainer Erdmann, Edgar Klose

Proceedings Volume 2388, (1995) https://doi.org/10.1117/12.208465

KEYWORDS: Molecules, Luminescence, Rhodamine, Photons, Bioalcohols, Molecular lasers, Time metrology, Signal detection, Electronics, Data processing

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Proceedings Article | 22 December 1994 Paper

Very compact all solid state fluorescence lifetime measurement system: preliminary results

Rainer Erdmann, Gerald Kell, Rolf Krahl, Uwe Ortmann, Wolfgang Becker, Joerg Enderlein, Edgar Klose

Proceedings Volume 2328, (1994) https://doi.org/10.1117/12.197519

KEYWORDS: Picosecond phenomena, Luminescence, Sensors, Semiconductor lasers, Diodes, Electronics, Signal detection, Ultrafast phenomena, Single photon, Monochromators

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Proceedings Article | 21 July 1994 Paper

New compact TCSPC apparatus based on sub 15 ps laserdiodes: preliminary results

Rainer Erdmann, Gerald Kell, Wolfgang Becker, Edgar Klose

Proceedings Volume 2136, (1994) https://doi.org/10.1117/12.180798

KEYWORDS: Picosecond phenomena, Diodes, Luminescence, Ultrafast phenomena, Single photon, Laser development, Pulsed laser operation, Stray light, Sapphire lasers, Data acquisition

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Proceedings Article | 24 June 1993 Paper

Investigations in high-speed DNA sequencing

Rainer Erdmann, Claus Seidel, F. Schubert, Edgar Klose

Proceedings Volume 1895, (1993) https://doi.org/10.1117/12.146726

KEYWORDS: Luminescence, Diagnostics, Absorption, Single photon, Molecules, Picosecond phenomena, Signal detection, Fluorescence spectroscopy, Data analysis, Second-harmonic generation

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Showing 5 of 81 publications

Proceedings Volume Editor (16)

SPIE Conference Volume | 29 March 2024

Single Molecule Spectroscopy and Superresolution Imaging XVII

SPIE Conference Volume | 24 April 2023

Single Molecule Spectroscopy and Superresolution Imaging XVI

SPIE Conference Volume | 13 April 2022

Single Molecule Spectroscopy and Superresolution Imaging XV

SPIE Conference Volume | 9 April 2021

Single Molecule Spectroscopy and Superresolution Imaging XIV

SPIE Conference Volume | 3 March 2020

Single Molecule Spectroscopy and Superresolution Imaging XIII

Showing 5 of 16 publications

Conference Committee Involvement (18)

Single Molecule Spectroscopy and Superresolution Imaging XVIII

25 January 2025 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XVII

27 January 2024 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XVI

28 January 2023 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XV

22 January 2022 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XIV

6 March 2021 | Online Only, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XIII

1 February 2020 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XII

2 February 2019 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XI

27 January 2018 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging X

28 January 2017 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging IX

13 February 2016 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging VIII

7 February 2015 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging VII

1 February 2014 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging VI

2 February 2013 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging V

21 January 2012 | San Francisco, California, United States

Single Molecule Spectroscopy and Imaging IV

22 January 2011 | San Francisco, California, United States

Single Molecule Spectroscopy and Imaging III

23 January 2010 | San Francisco, California, United States

Single Molecule Spectroscopy and Imaging II

24 January 2009 | San Jose, California, United States

Single Molecule Spectroscopy and Imaging

19 January 2008 | San Jose, California, United States

Showing 5 of 18 Conference Committees

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