Prof. Richard B. Fair Profile

Prof. Richard B. Fair

Professor at Duke Univ

SPIE Involvement:

Author

Proceedings Article | 2 March 2022 Presentation + Paper

Enhanced fluorescent detection of nucleosomes using functionalized magnetic beads on a digital microfluidic device

Yaas Bigdeli, Shruti Preetam, Kristen Scott, Zhanwei Zhong, Tung-Che Liang, Zachary Tini, Krishnendu Chakrabarty, Richard Fair

Proceedings Volume 11955, 1195506 (2022) https://doi.org/10.1117/12.2620556

KEYWORDS: Magnetism, Microfluidics, Luminescence, Fluorescence spectroscopy, Electrodes, Semiconducting wafers, Proteins, Microscopes, Control systems

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Proceedings Article | 5 March 2021 Presentation + Paper

Fluorescent detection of nucleosomes using functionalized magnetic beads on a digital microfluidic device

Yaas Bigdeli, Shruti Preetam, Kristen Scott, Zhanwei Zhong, Tung-Che Liang, Krishnendu Chakrabarty, Richard Fair

Proceedings Volume 11637, 116370L (2021) https://doi.org/10.1117/12.2578339

KEYWORDS: Magnetism, Microfluidics, Proteins, Pathology, Macromolecules, Fluoroscopy, Cancer

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Proceedings Article | 8 December 2004 Paper

Integrated chemical/biochemical sample collection, pre-concentration, and analysis on a digital microfluidic lab-on-a-chip platform

Richard Fair, A. Khlystov, Vijay Srinivasan, Vamsee Pamula, Kathryn Weaver

Proceedings Volume 5591, (2004) https://doi.org/10.1117/12.581955

KEYWORDS: Microfluidics, Aerosols, Lab on a chip, Statistical analysis, Electrodes, Chemical analysis, Interfaces, Particles, Absorbance, Explosives

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Proceedings Article | 8 December 2004 Paper

Protein stamping for MALDI mass spectrometry using an electrowetting-based microfluidic platform

Vijay Srinivasan, Vamsee Pamula, Phil Paik, Richard Fair

Proceedings Volume 5591, (2004) https://doi.org/10.1117/12.580145

KEYWORDS: Proteins, Microfluidics, Liquids, Calibration, Electrodes, Mass spectrometry, Adsorption, Statistical analysis, Silicon, Glasses

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MALDI-MS (matrix-assisted laser desorption/ionization mass spectrometry) is one of the most commonly used techniques for protein analysis. In conventional systems sample preparation is typically done in well-plates and transferred onto a MALDI target by robotic systems, which are complex, huge, expensive and slow. In this paper, we present a droplet-based microfluidic interface to transfer protein samples from a well-plate format onto a MALDI target for MS analysis. The droplets are actuated using the electrowetting phenomenon, and are immersed in silicone oil which prevents non-specific adsorption and enables the manipulation of high concentrations of proteins. Droplet transport and droplet formation were evaluated as a function of protein concentration using bovine serum albumin (BSA) as a test system. Droplet transport was possible for BSA concentrations up to 10mg/mL which is three orders of magnitude higher than previously reported results on handling proteins by electrowetting. Droplet formation from on-chip reservoirs, using only electrowetting forces and no external pressure assistance, was possible up to concentrations of 0.01mg/mL. An interface between a well-plate format and the electrowetting chip, and a scheme to passively stamp droplets onto a target substrate was then designed and tested by stamping BSA solutions. In two separate experiments 3.6fmoles and 16fmoles of BSA were stamped onto a glass slide using 0.001mg/mL and 0.01mg/mL samples respectively. A protein mixture with known constituents (ABI 4700 proteomics analyzer calibration solution) was stamped onto a MALDI plate and the individual proteins were correctly identified in the mass spectrum obtained using MALDI-TOF MS. The preliminary results establish the feasibility of using an electrowetting-based microfluidic system to handle proteins especially for protein stamping applications. The proposed system has a small footprint, is easy to control, and is very fast compared to conventional robotic systems. In addition, there are no moving parts and the associated mechanical reliability issues. Future work involves scaling to a larger number of samples and integration of sample preparation steps on-chip.

Proceedings Article | 22 August 2000 Paper

Detection of dissolved TNT and DNT in soil with a MEMS explosive particle detector

Vamsee Pamula, Richard Fair

Proceedings Volume 4038, (2000) https://doi.org/10.1117/12.396283

KEYWORDS: Particles, Explosives, Sensors, Microelectromechanical systems, Land mines, Explosives detection, Soil contamination, Silicon, Chromatography, Nose

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Proceedings Article | 2 August 1999 Paper

Detection of nanogram explosive particles with a MEMS sensor

Vamsee Pamula, Richard Fair

Proceedings Volume 3710, (1999) https://doi.org/10.1117/12.357055

KEYWORDS: Particles, Sensors, Explosives, Land mines, Microelectromechanical systems, Explosives detection, Silicon, Lamps, Gold, Ceramics

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Proceedings Article | 4 September 1998 Paper

MEMS devices for detecting the presence of explosive material residues in mine fields

Richard Fair, Michael Pollack, Vamsee Pamula

Proceedings Volume 3392, (1998) https://doi.org/10.1117/12.324214

KEYWORDS: Particles, Explosives, Transducers, Sensors, Land mines, Microelectromechanical systems, Silicon, Explosives detection, Infrared radiation, Ultrasonics

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Proceedings Article | 22 July 1997 Paper

MEMS-based explosive particle detection and remote particle stimulation

Richard Fair, Vamsee Pamula, Michael Pollack

Proceedings Volume 3079, (1997) https://doi.org/10.1117/12.280896

KEYWORDS: Particles, Explosives, Sensors, Land mines, Ultrasonics, Explosives detection, Microelectromechanical systems, Transducers, Silicon, Ultrasonography

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Proceedings Article | 19 June 1997 Paper

Integrated force array: interface to external systems

Stephen Bobbio, Stephen Smith, Scott Goodwin-Johansson, Richard Fair, Thomas DuBois, Farid Tranjan, John Hudak, R. Gupta, Hussein Makki

Proceedings Volume 3046, (1997) https://doi.org/10.1117/12.276614

KEYWORDS: Adhesives, Ferroelectric materials, Transducers, Metals, Picture Archiving and Communication System, Scanners, Thin films, Ultrasonography, Interfaces, Photoresist materials

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Proceedings Article | 20 May 1996 Paper

Integrated force array: positioning drive applications

Stephen Bobbio, Scott Goodwin-Johansson, Thomas DuBois, Farid Tranjan, Stephen Smith, Richard Fair, Christian Ball, James Jacobson, Charles Bartlett, Nadeem Eleyan, Hussein Makki, R. Gupta

Proceedings Volume 2722, (1996) https://doi.org/10.1117/12.240436

KEYWORDS: Ferroelectric materials, Transducers, Capacitors, Metals, Scanners, Ultrasonography, Prototyping, Connectors, Domes, Thin films

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Showing 5 of 10 publications

Conference Committee Involvement (1)

Lab-on-a-Chip: Platforms, Devices, and Applications

26 October 2004 | Philadelphia, Pennsylvania, United States

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